Usefulness of CHROMagar Candida Medium, Biochemical Methods - API ID32C and VITEK 2 Compact and Two MALDI-TOF MS Systems for Candida spp. Identification

被引:19
作者
Stefaniuk, Elzbieta [1 ,2 ]
Baraniak, Anna [3 ]
Fortuna, Monika [1 ]
Hryniewicz, Waleria [1 ,2 ]
机构
[1] Ctr Qual Control Microbiol, Warsaw, Poland
[2] Natl Med Inst, Dept Epidemiol & Clin Microbiol, Warsaw, Poland
[3] Natl Med Inst, Dept Mol Microbiol, Warsaw, Poland
关键词
Candida spp. - identification; chromogenic medium and biochemical methods; MALDI-TOF MS; DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; INTENSIVE-CARE; DUBLINIENSIS; YEAST; ALBICANS; FLUCONAZOLE;
D O I
10.5604/17331331.1197283
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This study was conducted to compare of the yeasts identification results obtained with two new systems using the MALDI-TOF MS technique with the ones obtained using the routine identification methods of Candida spp. in clinical microbiology laboratories. All 124 Candida spp. isolates were recovered from the routine examination of clinical specimens in microbiological laboratories and collected in the Centre of Quality Control in Microbiology in Warsaw (Poland). Our findings confirm the high agreement (98%) of fungal identification using the standard, biochemistry laboratory methods and mass spectrometry technique.
引用
收藏
页码:111 / 114
页数:4
相关论文
共 19 条
  • [11] Replacement of Candida albicans with C-dubliniensis in human immunodeficiency virus-infected patients with oropharyngeal candidiasis treated with fluconazole
    Martinez, M
    López-Ribot, JL
    Kirkpatrick, WR
    Coco, BJ
    Bachmann, SP
    Patterson, TF
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (09) : 3135 - 3139
  • [12] Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry vs conventional methods in the identification of Candida non-albicans
    Martinez-Lamas, Lucia
    Luisa Perez del Molino, Maria
    Pardo, Fernanda
    Varela, Eduardo
    Jose Regueiro, Benito
    [J]. ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA, 2011, 29 (08): : 568 - 572
  • [13] Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Direct Bacterial Identification from Positive Blood Culture Pellets
    Prod'hom, Guy
    Bizzini, Alain
    Durussel, Christian
    Bille, Jacques
    Greub, Gilbert
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (04) : 1481 - 1483
  • [14] Rapid and reliable MALDI-TOF mass spectrometry identification of Candida non-albicans isolates from bloodstream infections
    Pulcrano, Giovanna
    Iula, Dora Vita
    Vollaro, Antonio
    Tucci, Alessandra
    Cerullo, Monica
    Esposito, Matilde
    Rossano, Fabio
    Catania, Maria Rosaria
    [J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2013, 94 (03) : 262 - 266
  • [15] Raut SH, 2009, INDIAN J MED MICROBI, V27, P55
  • [16] Development of simultaneous resistance to fluconazole in Candida albicans and Candida dubliniensis in a patient with AIDS
    Ruhnke, M
    Schmidt-Westhausen, A
    Morschhäuser, J
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2000, 46 (02) : 291 - 295
  • [17] Usefulness of MALDI-TOF Mass Spectrometry for Routine Identification of Candida Species in a Resource-Poor Setting
    Sow, Doudou
    Fall, Becaye
    Ndiaye, Magatte
    Ba, Bissoume Sambe
    Sylla, Khadime
    Tine, Roger
    Lo, Aminata Colle
    Abiola, Annie
    Wade, Boubacar
    Dieng, Therese
    Dieng, Yemou
    Ndiaye, Jean Louis
    Hennequin, Christophe
    Gaye, Oumar
    Faye, Babacar
    [J]. MYCOPATHOLOGIA, 2015, 180 (3-4) : 173 - 179
  • [18] Candida dubliniensis:: Characteristics and identification
    Sullivan, D
    Coleman, D
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (02) : 329 - 334
  • [19] High-Throughput Identification of Bacteria and Yeast by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry in Conventional Medical Microbiology Laboratories
    van Veen, S. Q.
    Claas, E. C. J.
    Kuijper, Ed J.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (03) : 900 - 907