Usefulness of CHROMagar Candida Medium, Biochemical Methods - API ID32C and VITEK 2 Compact and Two MALDI-TOF MS Systems for Candida spp. Identification

被引:19
作者
Stefaniuk, Elzbieta [1 ,2 ]
Baraniak, Anna [3 ]
Fortuna, Monika [1 ]
Hryniewicz, Waleria [1 ,2 ]
机构
[1] Ctr Qual Control Microbiol, Warsaw, Poland
[2] Natl Med Inst, Dept Epidemiol & Clin Microbiol, Warsaw, Poland
[3] Natl Med Inst, Dept Mol Microbiol, Warsaw, Poland
关键词
Candida spp. - identification; chromogenic medium and biochemical methods; MALDI-TOF MS; DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; INTENSIVE-CARE; DUBLINIENSIS; YEAST; ALBICANS; FLUCONAZOLE;
D O I
10.5604/17331331.1197283
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This study was conducted to compare of the yeasts identification results obtained with two new systems using the MALDI-TOF MS technique with the ones obtained using the routine identification methods of Candida spp. in clinical microbiology laboratories. All 124 Candida spp. isolates were recovered from the routine examination of clinical specimens in microbiological laboratories and collected in the Centre of Quality Control in Microbiology in Warsaw (Poland). Our findings confirm the high agreement (98%) of fungal identification using the standard, biochemistry laboratory methods and mass spectrometry technique.
引用
收藏
页码:111 / 114
页数:4
相关论文
共 19 条
  • [1] Epidemiological trends in nosocomial candidemia in intensive care
    Bassetti, M
    Righi, E
    Costa, A
    Fasce, R
    Molinari, MP
    Rosso, R
    Pallavicini, FB
    Viscoli, C
    [J]. BMC INFECTIOUS DISEASES, 2006, 6 (1)
  • [2] Incidence of candidaemia and relationship with fluconazole use in an intensive care unit
    Bassetti, Matteo
    Ansaldi, Filippo
    Nicolini, Laura
    Malfatto, Emanuele
    Molinari, Maria Pia
    Mussap, Michele
    Rebesco, Barbara
    Pallavicini, Franco Bobbio
    Icardi, Giancarlo
    Viscoli, Claudio
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2009, 64 (03) : 625 - 629
  • [3] Phenotypic methods and commercial systems for the discrimination between C-albicans and C-dubliniensis
    Campanha, NH
    Neppelenbroek, KH
    Spolidorio, DMP
    Spolidorio, LC
    Pavarina, AC
    [J]. ORAL DISEASES, 2005, 11 (06) : 392 - 398
  • [4] Charles MVP, 2015, PHARMACOGN RES, V7, P69, DOI [10.4103/0974-8490.150528, 10.4103/0973-7847.156333]
  • [5] Clark RB., 2009, Cumitech 31A, Verification and validation of procedures in the clinical microbiology laboratory
  • [6] Yeast identification in the clinical microbiology accepted 15 August 2000 laboratory: phenotypical methods
    Freydiere, AM
    Guinet, R
    Boiron, P
    [J]. MEDICAL MYCOLOGY, 2001, 39 (01) : 9 - 33
  • [7] Evaluation of the VITEK 2 system for rapid identification of yeasts and yeast-like organisms
    Graf, B
    Adam, T
    Zill, E
    Göbel, UB
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (05) : 1782 - 1785
  • [8] Detection of Candida dubliniensis in oropharyngeal samples from human immunodeficiency virus-infected patients in North America by primary CHROMagar Candida screening and susceptibility testing of isolates
    Kirkpatrick, WR
    Revankar, SG
    McAtee, RK
    Lopez-Ribot, JL
    Fothergill, AW
    McCarthy, DI
    Sanche, SE
    Cantu, RA
    Rinaldi, MG
    Patterson, TF
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (10) : 3007 - 3012
  • [9] Emergence of Candida parapsilosis as the predominant species causing candidemia in children
    Levy, I
    Rubin, LG
    Vasishtha, S
    Tucci, V
    Sood, SK
    [J]. CLINICAL INFECTIOUS DISEASES, 1998, 26 (05) : 1086 - 1088
  • [10] Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Fast and Reliable Identification of Clinical Yeast Isolates
    Marklein, G.
    Josten, M.
    Klanke, U.
    Mueller, E.
    Horre, R.
    Maier, T.
    Wenzel, T.
    Kostrzewa, M.
    Bierbaum, G.
    Hoerauf, A.
    Sahl, H. -G.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (09) : 2912 - 2917