Identification of cyst forming nematodes of the genus Heterodera (Nematoda: Heteroderidae) based on the ribosomal DNA-RFLP

被引：137
作者：
Subbotin, SA
Waeyenberge, L
Moens, M
机构：
[1] Agr Res Ctr, B-9820 Merelbeke, Belgium
[2] Russian Acad Sci, Inst Parasitol, Moscow 117071, Russia
来源：
NEMATOLOGY | 2000年 / 2卷
关键词：
heterogeneity; ITS regions; Meloidodera alni;
D O I：
10.1163/156854100509042
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
Amplified ITS region products of rDNA from 25 valid species and one unidentified species from the genus Heterodera and from Meloidodera alni were digested by 26 restriction enzymes. A combination of seven enzymes clearly separated the agriculturally most important species from each other and from their sibling species. Species specific digestion profiles of ITS regions and a table with approximate sizes of digested fragments for several identification enzymes are given. Heterogeneity of ITS regions was revealed for some Cyst forming nematode species.
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页码：153 / 164
页数：12
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