Inflammasome assembly in the chorioamniotic membranes during spontaneous labor at term

被引：31

作者：

Gomez-Lopez, Nardhy ^{[1
,2
,3
,4
]}

Romero, Roberto ^{[2
,3
,5
,6
,7
]}

Xu, Yi ^{[1
,2
,3
]}

Garcia-Flores, Valeria ^{[1
,2
,3
]}

Leng, Yaozhu ^{[1
,2
,3
]}

Panaitescu, Bogdan ^{[1
,2
,3
]}

Miller, Derek ^{[1
,2
,3
]}

Abrahams, Vikki M. ^{[8
]}

Hassan, Sonia S. ^{[1
,2
,3
]}

机构：

[1] Wayne State Univ, Sch Med, Dept Obstet & Gynecol, Detroit, MI 48201 USA

[2] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Perinatol Res Branch, Program Perinatal Res & Obstet, Div Intramural Res,NICHD,NIH,DHHS, Bethesda, MD USA

[3] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Perinatol Res Branch, Program Perinatal Res & Obstet, Div Intramural Res,NICHD,NIH,DHHS, Detroit, MI USA

[4] Wayne State Univ, Sch Med, Dept Immunol & Microbiol, Detroit, MI 48201 USA

[5] Univ Michigan, Dept Obstet & Gynecol, Ann Arbor, MI 48109 USA

[6] Michigan State Univ, Dept Epidemiol & Biostat, E Lansing, MI 48824 USA

[7] Wayne State Univ, Ctr Mol Med & Genet, Detroit, MI USA

[8] Yale Univ, Sch Med, Dept Obstet Gynecol & Reprod Sci, New Haven, CT USA

来源：

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 2017年 / 77卷 / 05期

基金：

美国国家卫生研究院;

关键词：

acute chorioamnionitis; apoptosis-associated speck-like protein containing a CARD (ASC); ASCspeck; caspase-1; interleukin-1; beta; intra-amniotic infection/inflammation; INTERLEUKIN-1 RECEPTOR ANTAGONIST; TUMOR-NECROSIS-FACTOR; NF-KAPPA-B; AMNIOTIC-FLUID INTERLEUKIN-6; GAMMA-INDUCING FACTOR; PRETERM LABOR; IMMUNE-RESPONSE; CELL-DEATH; INTRAAMNIOTIC INFECTION; MICROBIAL INVASION;

D O I：

10.1111/aji.12648

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Problem: Inflammasome activation requires two steps: priming and assembly of the multimeric complex. The second step includes assembly of the sensor molecule and adaptor protein ASC (an apoptosis-associated speck-like protein containing a CARD), which results in ASC speck formation and the recruitment of caspase (CASP)-1. Herein, we investigated whether there is inflammasome assembly in the chorioamniotic membranes and choriodecidual leukocytes from women who underwent spontaneous labor at term. Method of Study: Using in situ proximity ligation assays, ASC/CASP-1 complexes were determined in the chorioamniotic membranes from women who delivered at term without labor or underwent spontaneous labor at term with or without acute histologic chorioamnionitis (n=10-11 each). Also, ASC speck formation was determined by flow cytometry in the choriodecidual leukocytes isolated from women who delivered at term with or without spontaneous labor (n=9-12 each). Results: (i) ASC/CASP-1 complexes were detected in the chorioamniotic membranes; (ii) ASC/CASP-1 complexes were greater in the chorioamniotic membranes from women who underwent spontaneous labor at term than in those without labor; (iii) ASC/CASP-1 complexes were even more abundant in the chorioamniotic membranes from women who underwent spontaneous labor at term with acute histologic chorioamnionitis than in those without this placental lesion; (iv) ASC speck formation was detected in the choriodecidual leukocytes; and (v) ASC speck formation was greater in the choriodecidual leukocytes isolated from women who underwent spontaneous labor at term than in those without labor. Conclusion: There is inflammasome assembly in the chorioamniotic membranes and choriodecidual leukocytes during spontaneous labor at term.

引用

页数：10

共 100 条

[1] NALP3 forms an IL-lβ-Processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder [J].