Intraspecific variation in Cannabis sativa L. based on intergenic spacer region of chloroplast DNA

被引：0

作者：

Kohjyouma, M

Lee, IJ

Iida, O

Kurihara, K

Yamada, K

Makino, Y

Sekita, S

Satake, M

机构：

[1] Natl Inst Hlth Sci, Izu Expt Stn Med Plants, Shizuoka 4150303, Japan

[2] Natl Inst Hlth Sci, Div Pharmacognosy & Phytochem, Setagaya Ku, Tokyo 1588501, Japan

[3] Kanto Shinetsu Reg Narcot Control Off, Meguro Ku, Tokyo 1530061, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2000年 / 23卷 / 06期

关键词：

Cannabis sativa L; chloroplast DNA; intergenic spacer; intraspecific variation; PCR-SSCP;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We analyzed the nucleotide sequences of the non-coding region of chloroplast DNA: the intergenic spacer between trnL (UAA) 3'exon and trnF (GAA). Two kinds of sequence, "type-1" and "type-2," were detected in 33 populations of Cannabis sativa. The length of the "type-1" fragment was 354 bp. In contrast, the "type-2" fragment from 3 populations was 353 bp long, with only one base deletion compared to "type-1" The fragment length from Humulus lupulus was 353 bp with a 1-bp deletion, and ten 1-bp substitutions compared to the sequences from C. sativa "type-1". Furthermore, we could clearly identify differences between C. saliva and H. lupulus using single-strand conformation polymorphism of PCR products (PCR-SSCP) analysis.

引用

页码：727 / 730

页数：4

共 50 条

[21] Intraspecific phenotypic variation associated with nuclear DNA content in Lolium perenne L.
Sugiyama, S
Yamaguchi, K
Yamada, T
EUPHYTICA, 2002, 128 (02) : 145 - 151
[22] Intraspecific phenotypic variation associated with nuclear DNA content in Lolium perenne L.
Shuichi Sugiyama
Kenichi Yamaguchi
Toshihiko Yamada
Euphytica, 2002, 128 : 145 - 151
[23] Variation of Wx microsatellite allele, waxy allele distribution and differentiation of chloroplast DNA in a collection of Thai rice (Oryza sativa L.)
Prathepha, P
Baimai, V
EUPHYTICA, 2004, 140 (03) : 231 - 237
[24] A PCR-based method for assaying molecular variation in corals based on RFLP analysis of the ribosomal intergenic spacer region
Smith, C
Chen, CA
Yang, HP
Miller, DJ
MOLECULAR ECOLOGY, 1997, 6 (07) : 683 - 685
[25] PCR amplification and characterization of the intergenic spacer region of the ribosomal DNA in Pyrenophora graminea
Pecchia, S
Mercatelli, E
Vannacci, G
FEMS MICROBIOLOGY LETTERS, 1998, 166 (01) : 21 - 27
[26] CANNABIS SATIVA L. - CULTIVATION AND QUALITY OF RAW MATERIAL
Zuk-Golaszewska, Krystyna
Golaszewski, Janusz
JOURNAL OF ELEMENTOLOGY, 2018, 23 (03): : 971 - 984
[27] Hemp (Cannabis sativa L.) as an Environmentally Friendly Energyplant
Poisa, Liena
Adamovics, Aleksandrs
ENVIRONMENTAL AND CLIMATE TECHNOLOGIES, 2010, 5 (01) : 80 - 85
[28] Chloroplast DNA variation in the Turnera sidoides L. complex (Turneraceae):: biogeographical implications
Speranza, P. R.
Seijo, J. G.
Grela, I. A.
Neffa, V. G. Solis
JOURNAL OF BIOGEOGRAPHY, 2007, 34 (03) : 427 - 436
[29] Absence of geographical structure of chloroplast DNA variation in sallow, Salix caprea L.
A E Palmé
V Semerikov
M Lascoux
Heredity, 2003, 91 : 465 - 474
[30] Absence of geographical structure of chloroplast DNA variation in sallow, Salix caprea L.
Palmé, AE
Semerikov, V
Lascoux, M
HEREDITY, 2003, 91 (05) : 465 - 474

← 1 2 3 4 5 →