Stress-induced changes of neurosteroid profiles in rat brain and plasma under immobilized condition

被引:20
作者
Park, Myeong Hyeon [1 ,2 ]
Rehman, Shaheed Ur [3 ]
Kim, In Sook [1 ,2 ]
Choi, Min Sun [1 ,2 ]
Yoo, Hye Hyun [1 ,2 ]
机构
[1] Hanyang Univ, Inst Pharmaceut Sci & Technol, Gyeonggi Do 15588, South Korea
[2] Hanyang Univ, Coll Pharm, Gyeonggi Do 15588, South Korea
[3] COMSATS Inst Informat Technol, Dept Pharm, Abbottabad, Pakistan
基金
新加坡国家研究基金会;
关键词
Neurosteroids; LC-MS/MS; Brain; Plasma; Immobilization stress; IONIZATION-MASS SPECTROMETRY; D-ASPARTATE RECEPTORS; NEUROACTIVE STEROIDS; LIQUID; TESTOSTERONE; PREGNENOLONE; ETHANOL; SITES;
D O I
10.1016/j.jpba.2017.02.007
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this study, various neurosteroids in brain and plasma were simultaneously determined using liquid chromatography-tandem mass spectrometry and their profile changes in a stress-induced rats were investigated. The investigated neurosteroids are as follows: progesterone (P4), 5 alpha-dihydroprogesterone (5 alpha-DHP), 5 beta-dihydroprogesterone, estrone, androstenedione (AE), cortisol, cortisone, corticosterone (CORT), dehydroepiandrosterone (DHEA), pregnanolone (3 alpha,5 beta-THP), allopregnanolone (ALLO), 11-deoxycorticosterone (DOC), 11-deoxycortisol, pregnenolone (PREG), and 5 alpha/5 beta-tetrahydrodeoxycorticosterone (5 alpha/5 beta-THDOC). Brain and plasma samples were processed using solid-phase extraction, with methanol and acetic acid (99:1), and derivatized with a hydroxylamine reagent. Separation was achieved within 13 min at a flow rate of 0.4 mL/min with a C18 column (3.0 x 50 mm, 2.7 mu m). The triple quadrupole mass spectrometer was operated in the positive electrospray ionization mode. Using this method, the neurosteroid level variation was quantitated and investigated in the brain and plasma upon immobilization stress in rats. As a result, AE, CORT, DOC, P4, 5 alpha-DHP, 5 alpha/5 beta-THDOC, DHEA, 3 alpha,5 beta-THP, ALLO, and PREG levels were significantly altered in both the brain and plasma samples when stress was induced. These findings demonstrated that stress leads to the alteration of the GABAergic neurosteroid profile. The present results will be helpful for furthering an understanding of the role of neurosteroids in stressed conditions. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:92 / 99
页数:8
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