Permeability Study of Polyphenols Derived from a Phenolic-Enriched Hibiscus sabdariffa Extract by UHPLC-ESI-UHR-Qq-TOF-MS

被引：29

作者：

Borras-Linares, Isabel ^{[1
,2
]}

Herranz-Lopez, Maria ^{[3
]}

Barrajon-Catalan, Enrique ^{[3
]}

Arraez-Roman, David ^{[1
,2
]}

Gonzalez-Alvarez, Isabel ^{[4
]}

Bermejo, Marival ^{[4
]}

Fernandez Gutierrez, Alberto ^{[1
,2
]}

Micol, Vicente ^{[3
]}

Segura-Carretero, Antonio ^{[1
,2
]}

机构：

[1] Univ Granada, Fac Sci, Dept Analyt Chem, E-18071 Granada, Spain

[2] Res & Dev Funct Food Ctr, Granada 18016, Spain

[3] Miguel Hernandez Univ, IBMC, Alicante 03202, Spain

[4] Univ Miguel Hernandez, Dept Engn, Pharmacokinet & Pharmaceut Technol Area, Alicante 03550, Spain

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2015年 / 16卷 / 08期

关键词：

Hibiscus sabdariffa; phenolic compounds; flavonoids; Caco-2; cells; UHPLC-ESI-UHR-Qq-TOF-MS; CHLOROGENIC ACID QUINONE; GASTRIC-CARCINOMA CELLS; LOWERS BLOOD-PRESSURE; TRANSEPITHELIAL TRANSPORT; IN-VITRO; AQUEOUS EXTRACT; HUMAN PLASMA; ANTIOXIDANT; L; QUERCETIN;

D O I：

10.3390/ijms160818396

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous findings on the capacity of Hibiscus sabdariffa (HS) polyphenols to ameliorate metabolic disturbances justify the necessity of studies oriented to find the potential metabolites responsible for such an effect. The present study examined the intestinal epithelial membrane permeability of polyphenols present in a phenolic-enriched Hibiscus sabdariffa extract (PEHS), free and encapsulated, using the Caco-2 cell line. Additionally, selected polyphenols (quercetin, quercetin-3-glucoside, quercetin-3-glucuronide, and N-feruloyltyramine) were also studied in the same absorption model. The powerful analytical platform used ultra-high-performance liquid chromatography coupled with ultra-high-resolution quadrupole time-of-flight mass spectrometry (UHPLC-ESI-UHR-Qq-TOF-MS), and enabled the characterization of seven new compounds in PEHS. In the permeation study, only a few compounds were able to cross the cell monolayer and the permeability was lower when the extract was in an encapsulated form. Pure compounds showed a moderate absorption in all cases. Nevertheless, these preliminary results may need further research to understand the complete absorption mechanism of Hibiscus polyphenols.

引用

页码：18396 / 18411

页数：16

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