Ultrahigh-Throughput Improvement and Discovery of Enzymes Using Droplet-Based Microfluidic Screening

被引：42

作者：

Autour, Alexis ^{[1
]}

Ryckelynck, Michael ^{[1
]}

机构：

[1] Univ Strasbourg, CNRS, Architecture & Reactivite ARN, UPR 9002, F-67000 Strasbourg, France

来源：

MICROMACHINES | 2017年 / 8卷 / 04期

关键词：

droplet-based microfluidics; high-throughput screening; enzyme improvement; directed evolution; single-cell; FLUORINATED PICKERING EMULSIONS; POLYMERASE-CHAIN-REACTION; CELL-LYSATE ASSAYS; DIRECTED EVOLUTION; SINGLE CELLS; CONCENTRATION GRADIENT; MOLECULAR-TRANSPORT; PICOLITER DROPLETS; PROTEIN EXPRESSION; FLOW;

D O I：

10.3390/mi8040128

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Enzymes are extremely valuable tools for industrial, environmental, and biotechnological applications and there is a constant need for improving existing biological catalysts and for discovering new ones. Screening microbe or gene libraries is an efficient way of identifying new enzymes. In this view, droplet-based microfluidics appears to be one of the most powerful approaches as it allows inexpensive screenings in well-controlled conditions and an ultrahigh-throughput regime. This review aims to introduce the main microfluidic devices and concepts to be considered for such screening before presenting and discussing the latest successful applications of the technology for enzyme discovery.

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