Ultrahigh-Throughput Improvement and Discovery of Enzymes Using Droplet-Based Microfluidic Screening

被引：42

作者：

Autour, Alexis ^{[1
]}

Ryckelynck, Michael ^{[1
]}

机构：

[1] Univ Strasbourg, CNRS, Architecture & Reactivite ARN, UPR 9002, F-67000 Strasbourg, France

来源：

MICROMACHINES | 2017年 / 8卷 / 04期

关键词：

droplet-based microfluidics; high-throughput screening; enzyme improvement; directed evolution; single-cell; FLUORINATED PICKERING EMULSIONS; POLYMERASE-CHAIN-REACTION; CELL-LYSATE ASSAYS; DIRECTED EVOLUTION; SINGLE CELLS; CONCENTRATION GRADIENT; MOLECULAR-TRANSPORT; PICOLITER DROPLETS; PROTEIN EXPRESSION; FLOW;

D O I：

10.3390/mi8040128

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Enzymes are extremely valuable tools for industrial, environmental, and biotechnological applications and there is a constant need for improving existing biological catalysts and for discovering new ones. Screening microbe or gene libraries is an efficient way of identifying new enzymes. In this view, droplet-based microfluidics appears to be one of the most powerful approaches as it allows inexpensive screenings in well-controlled conditions and an ultrahigh-throughput regime. This review aims to introduce the main microfluidic devices and concepts to be considered for such screening before presenting and discussing the latest successful applications of the technology for enzyme discovery.

引用

页数：21

共 157 条

[1] Impact of inlet channel geometry on microfluidic drop formation [J].

Abate, A. R. ;

Poitzsch, A. ;

Hwang, Y. ;

Lee, J. ;

Czerwinska, J. ;

Weitz, D. A. .

PHYSICAL REVIEW E, 2009, 80 (02)

[2] High-throughput injection with microfluidics using picoinjectors [J].

Abate, Adam R. ;

Hung, Tony ;

Mary, Pascaline ;

Agresti, Jeremy J. ;

Weitz, David A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (45) :19163-19166

[3] Microfluidic sorting with high-speed single-layer membrane valves [J].

Abate, Adam R. ;

Agresti, Jeremy J. ;

Weitz, David A. .

APPLIED PHYSICS LETTERS, 2010, 96 (20)

[4] Ultrahigh-throughput screening in drop-based microfluidics for directed evolution [J].

Agresti, Jeremy J. ;

Antipov, Eugene ;

Abate, Adam R. ;

Ahn, Keunho ;

Rowat, Amy C. ;

Baret, Jean-Christophe ;

Marquez, Manuel ;

Klibanov, Alexander M. ;

Griffiths, Andrew D. ;

Weitz, David A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (09) :4004-4009

[5] High-throughput screening of enzyme libraries: Thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments [J].

Aharoni, A ;

Amitai, G ;

Bernath, K ;

Magdassi, S ;

Tawfik, DS .

CHEMISTRY & BIOLOGY, 2005, 12 (12) :1281-1289

[6] On-demand electrostatic droplet charging and sorting [J].

Ahn, Byungwook ;

Lee, Kangsun ;

Panchapakesan, Rajagopal ;

Oh, Kwang W. .

BIOMICROFLUIDICS, 2011, 5 (02)

[7] Dielectrophoretic manipulation of drops for high-speed microfluidic sorting devices [J].

Ahn, K ;

Kerbage, C ;

Hunt, TP ;

Westervelt, RM ;

Link, DR ;

Weitz, DA .

APPLIED PHYSICS LETTERS, 2006, 88 (02) :1-3

[8] Electrocoalescence of drops synchronized by size-dependent flow in microfluidic channels [J].

Ahn, Keunho ;

Agresti, Jeremy ;

Chong, Henry ;

Marquez, Manuel ;

Weitz, D. A. .

APPLIED PHYSICS LETTERS, 2006, 88 (26)

[9] Formation of dispersions using "flow focusing" in microchannels [J].

Anna, SL ;

Bontoux, N ;

Stone, HA .

APPLIED PHYSICS LETTERS, 2003, 82 (03) :364-366

[10] iSpinach: a fluorogenic RNA aptamer optimized for in vitro applications [J].

Autour, Alexis ;

Westhof, Eric ;

Ryckelynck, Michael .

NUCLEIC ACIDS RESEARCH, 2016, 44 (06) :2491-2500

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