Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results

被引：32

作者：

Waters, W. Ray ^{[1
]}

Vordermeier, H. Martin ^{[2
]}

Rhodes, Shelley ^{[2
]}

Khatri, Bhagwati ^{[2
]}

Palmer, Mitchell V. ^{[1
]}

Maggioli, Mayara F. ^{[1
]}

Thacker, Tyler C. ^{[1
]}

Nelson, Jeffrey T. ^{[3
]}

Thomsen, Bruce V. ^{[3
]}

Robbe-Austerman, Suelee ^{[3
]}

Garcia, Doris M. Bravo ^{[3
]}

Schoenbaum, Mark A. ^{[4
]}

Camacho, Mark S. ^{[5
]}

Ray, Jean S. ^{[6
]}

Esfandiari, Javan ^{[7
]}

Lambotte, Paul ^{[7
]}

Greenwald, Rena ^{[7
]}

Grandison, Adrian ^{[7
]}

Sikar-Gang, Alina ^{[7
]}

Lyashchenko, Konstantin P. ^{[7
]}

机构：

[1] ARS, Natl Anim Dis Ctr, USDA, Ames, IA USA

[2] Anim & Plant Hlth Agcy, TB Res Grp, Addlestone, Surrey, England

[3] APHIS, Natl Vet Serv Labs, USDA, Ames, IA USA

[4] APHIS, VS, USDA, Ft Collins, CO USA

[5] APHIS, VS, USDA, Raleigh, NC USA

[6] APHIS, VS, USDA, E Lansing, MI USA

[7] Chembio Diagnost Syst Inc, Medford, NY 11763 USA

来源：

BMC VETERINARY RESEARCH | 2017年 / 13卷

基金：

美国食品与农业研究所;

关键词：

Antibody; Bovine tuberculosis; Dual path platform; Multi-antigen print immunoassay; Tuberculin skin test; Mycobacterium bovis; MYCOBACTERIUM-BOVIS INFECTION; SEROLOGICAL DIAGNOSIS; RESPONSES; DISEASE; ASSAY; IMMUNOASSAY; ANERGY; ELISA;

D O I：

10.1186/s12917-017-1085-5

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Background: Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results: Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP (R)) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB free herds. Multi antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB. Conclusions: Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.

引用

页数：7

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