Covalent Capturing of Transient SUMO-SIM Interactions Using Unnatural Amino Acid Mutagenesis and Photocrosslinking

被引：16

作者：

Taupitz, Kim F. ^{[1
]}

Doerner, Wolfgang ^{[1
]}

Mootz, Henning D. ^{[1
]}

机构：

[1] Univ Munster, Inst Biochem, Dept Chem & Pharm, Wilhelm Klemm Str 2, D-48149 Munster, Germany

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2017年 / 23卷 / 25期

关键词：

crosslinker; genetic code expansion; post-translational modification; protein interaction; SUMO; ESCHERICHIA-COLI; BINDING MOTIF; GENETIC-CODE; PROTEIN; RECOGNITION; SPECIFICITY; INSIGHTS; COMPLEX; SIGNAL;

D O I：

10.1002/chem.201605619

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The weak interaction between the post-translational modifier SUMO (small ubiquitin-like modifier) and proteins containing the SUMO-interacting motif (SIM) poses limitations to the identification of interaction partners of SUMOylated proteins and to the mapping of the interfaces. To overcome these limitations, genetically encoded photocrosslinker amino acids were incorporated close to the SIM-interaction groove in human SUMO1. UV irradiation resulted in the desired covalent crosslinks both in a purified protein environment and in cell extracts.

引用

页码：5978 / 5982

页数：5

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