Detecting amplicons of loop-mediated isothermal amplification

被引：36

作者：

Shirato, Kazuya ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Virol 3, Lab Acute Resp Viral Dis & Cytokines, Musashimurayama, Tokyo, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2019年 / 63卷 / 10期

基金：

日本学术振兴会;

关键词：

detection; LAMP; QPrimer; QProbe; turbidity; RAPID DETECTION; LAMP ASSAY; VIRUS-INFECTION; DIAGNOSIS; INFLUENZA; GENOME; DNA; SEQUENCES;

D O I：

10.1111/1348-0421.12734

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Loop-mediated isothermal amplification (LAMP) assays are used to detect diverse pathogens. Initially, LAMP amplicons were detected using electrophoresis; later, real-time monitoring based on turbidity was developed to overcome the problem of contamination with environmental DNA. Recently, real-time monitoring of fluorescence signals using a quenching primer and probe has improved the reliability of amplification signals. Here, methods of detecting LAMP amplicons are reviewed.

引用

页码：407 / 412

页数：6

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