Specificity of odorant-binding proteins: a factor influencing the sensitivity of olfactory receptor-based biosensors

被引：26

作者：

Ko, Hwi Jin ^{[1
]}

Lee, Sang Hun ^{[1
]}

Oh, Eun Hae ^{[1
]}

Park, Tai Hyun ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Chem & Biol Engn, Seoul 151744, South Korea

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2010年 / 33卷 / 01期

关键词：

Odorant-binding protein; Olfactory receptor; HEK-293; cell; Olfactory receptor-based biosensor; SURFACE-PLASMON RESONANCE; LIGAND-BINDING; ENHANCEMENT; IMMOBILIZATION; FABRICATION; EXPRESSION; SYSTEM; RAT;

D O I：

10.1007/s00449-009-0348-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Odorant-binding proteins (OBPs) primarily function in the transport of hydrophobic odorants. In this study, OBPs originating from rat and pig were cloned into a mammalian expression vector, pcDNA3, and expressed in HEK-293 cells, and their specificity for odorants and olfactory receptors was examined. Results suggest that OBPs have a high affinity for the olfactory receptors when both the OBP and receptor originate from the same species. The rat OBPs were bound not only to the rat olfactory receptor I7 but also to the odorant specific to I7. The solubility of the odorant was increased by both OBP2 and OBP3, which originate from rat, but with different efficiencies. These results demonstrate that OBPs specifically interact with odorants as well as olfactory receptors, and these interactions can influence the sensitivity of olfactory receptor-based biosensors.

引用

页码：55 / 62

页数：8

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