Chemical characterization and gastroprotective effect of an isolated polysaccharide fraction from Bletilla striata against ethanol-induced acute gastric ulcer

被引：99
作者：
Zhang, Chen [1 ]
Gao, Fei [1 ]
Gan, Shuai [1 ]
He, Yanan [1 ]
Chen, Zhejie [2 ,3 ]
Liu, Xiaowei [4 ]
Fu, Chaomei [1 ]
Qu, Yan [1 ]
Zhang, Jinming [1 ]
机构：
[1] Chengdu Univ Tradit Chinese Med, Pharm Coll, Chengdu 611137, Sichuan, Peoples R China
[2] Univ Macau, State Key Lab Qual Res Chinese Med, Taipa, Macao, Peoples R China
[3] Univ Macau, Inst Chinese Med Sci, Taipa, Macao, Peoples R China
[4] Chengdu Univ, Sch Med, Chengdu 610106, Sichuan, Peoples R China
来源：
FOOD AND CHEMICAL TOXICOLOGY | 2019年 / 131卷
基金：
中国国家自然科学基金;
关键词：
Bletilla striata polysaccharides; Gastric injury; Oxidative stress; MAPK/ NF-kappa B pathway; OXIDATIVE STRESS; MUCOSAL INJURY; IN-VITRO; ANTIOXIDANT; ALCOHOL; PATHOGENESIS; INFLAMMATION; MECHANISMS; LESIONS; OPTIMIZATION;
D O I：
10.1016/j.fct.2019.05.047
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The chemical characterization and protective role against ethanol-induced gastric ulcerated rats of a polysaccharide fraction from Bletilla striata (BSP) collected by ultrafiltration membrane approach were evaluated. This BSP faction was consisted of mannose and glucose at a molar ratio of 2.4:1 approximately, with a molecular weight of 146 KDa. FT-IR, NMR and XRD spectra indicated that BSP faction contained alpha-Man and beta-Glc residues with low overall crystallinity. The polysaccharide exhibited significant scavenging activities of ABTS and FRAP, as well as non-toxicity against human gastric epithelial GES-1 cells. Oral administration with 100 mg/kg of BSP for 3 days continuously could significantly prevent the formation of ethanol-induced gastric mucosal lesion. It could also reduce the levels of pro-inflammatory cytokines, including TNF-alpha, IL-1 beta, IL-6, and IL-18, and MPO activity in gastric tissue. Additionally, the BSP faction exhibited antioxidant activity, increased the content of PEG(2) as a defensive factor, and suppressed MAPK/NF-kappa B signaling pathway in gastric tissue. These results indicated that the gastroprotective activity of BSP faction could be attributed to the reduction of pro-inflammatory cytokines and oxidative stress and the inhibition of MAPK/NF-kappa B pathways. Our results provided substantial evidence that BSP could be a promising phytomedicine for gastric ulcer prevention.
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页数：10
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