Electrophoresis of DNA in ionic liquid coated capillary

被引:70
作者
Qin, WD
Li, SFY [1 ]
机构
[1] Natl Univ Singapore, Dept Chem, Singapore 119260, Singapore
[2] CE Resources Pte Ltd, Singapore 139948, Singapore
关键词
D O I
10.1039/b208724c
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An ionic liquid (IL) coated capillary was prepared and investigated for DNA separation. The electroosmotic flow of the capillary was reversed between pH 4.5 and 9. Below 900 base pairs the larger DNA fragment suffered more retardation in the IL coated capillary due to the increasing charge density of the fragment with size. In the presence of 4% hydroxyethylcellulose, the PhiX174 DNA-Hae III digest fragments were baseline separated in both IL- and polyacrylamide-coated capillary except for the fragments of 271 and 281 base pairs; while the analysis time was shorter in the IL- coated capillary. Our experiments indicated that the IL- coated capillary could work stably in the run buffer for at least 96 h with no notable deterioration in performance.
引用
收藏
页码:37 / 41
页数:5
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