Characterization of a Plasmid-Encoded Resistance-Nodulation Division Efflux Pump in Klebsiella pneumoniae and Klebsiella quasipneumoniae from Patients in China

被引：15
作者：
He, Ruowen [1 ,2 ]
Yang, Yongqiang [1 ,2 ,3 ]
Wu, Yiping [1 ,2 ]
Zhong, Lan-Lan [1 ,2 ]
Yang, Yanxian [1 ,2 ]
Chen, Guanping [4 ]
Qin, Mingyang [5 ]
Liang, Xiaoxue [6 ]
Ahmed, Mohamed Abd El-Gawad El-Sayed [1 ,2 ,7 ]
Lin, Minmin [8 ]
Yan, Bin [9 ]
Xia, Yong [10 ]
Dai, Min [6 ]
Chen, Hongtao [11 ]
Tian, Guo-Bao [1 ,2 ,12 ]
机构：
[1] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Microbiol, Guangzhou, Peoples R China
[2] Sun Yat Sen Univ, Minist Educ, Key Lab Trop Dis Control, Guangzhou, Peoples R China
[3] Sun Yat Sen Univ, Sch Pharmaceut Sci Shenzhen, Guangzhou, Peoples R China
[4] Sun Yat Sen Univ, Sch Med, Guangzhou, Peoples R China
[5] Xinxiang Med Univ, Basic Med Coll, Xinxiang, Henan, Peoples R China
[6] Chengdu Med Coll, Sch Lab Med, Chengdu, Peoples R China
[7] Misr Univ Sci & Technol, Fac Pharmaceut Sci & Drug Mfg, Dept Microbiol & Immunol, 6th Of October City, Egypt
[8] Sun Yat Sen Univ, Dept Resp Med, Affiliated Hosp 5, Zhuhai, Peoples R China
[9] Guangzhou Women & Childrens Med Ctr, Dept Neonatal Surg, Guangzhou, Peoples R China
[10] Guangzhou Med Univ, Dept Clin Lab Med, Affiliated Hosp 3, Guangzhou, Peoples R China
[11] Sun Yat Sen Univ, Affiliated Hosp 5, Dept Clin Lab, Zhuhai, Peoples R China
[12] Xizang Minzu Univ, Sch Med, Xianyang, Shaanxi, Peoples R China
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2021年 / 65卷 / 02期
基金：
中国博士后科学基金; 中国国家自然科学基金;
关键词：
Klebsiella spp; efflux pump; tmexCD1-toprJ1; tigecycline; antimicrobial resistance; ANTIMICROBIAL RESISTANCE; DIVERSITY;
D O I：
10.1128/AAC.02075-20
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Two multidrug-resistant (MDR) mcr-1-harboring Klebsiella pneumoniae isolates from patients with urinary tract infections and one MDR Klebsiella quasipneumoniae isolate from a patient with bloodstream infection were identified to carry tmexCD1-toprJ1. The addition of the efflux pump inhibitor reduced the tigecycline MIC against all three isolates by 8- to 16-fold. pKQBSI104-1 was transferred from K. quasipneumoniae to Escherichia coli J53 via conjugation. The tmexCD1-toprJ1-carrying plasmids pKP15ZE495-1 (102,569 bp) and pKQBSI104-1 (121,996 bp) were completely sequenced and analyzed.
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页数：5
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