Generation of a DKK1 homozygous knockout human embryonic stem cell line WAe001-A-21 using CRISPR/Cas9

被引：2

作者：

Yang, Zhen ^{[1
,2
,3
,4
]}

Yuan, Fang ^{[1
,3
,4
]}

Wang, Ning ^{[1
,3
,4
]}

Liu, Yanli ^{[1
,2
,3
]}

Zhang, Jiaye ^{[1
,4
]}

Wei, Ruzhi ^{[1
,2
,3
,4
]}

Wu, FeiMa ^{[1
,3
,4
]}

Wu, Yuhang ^{[1
,3
,4
]}

You, Kai ^{[1
,3
,4
]}

Chen, Yan ^{[1
,3
,4
]}

Yang, Fan ^{[1
,3
,4
]}

Li, Yin-Xiong ^{[1
,2
,3
,4
]}

机构：

[1] Chinese Acad Sci, Inst Publ Hlth, Guangzhou Inst Biomed & Hlth, Guangzhou, Peoples R China

[2] Univ Chinese Acad Sci, Beijing, Peoples R China

[3] Chinese Acad Sci, South China Inst Stem Cell Biol & Regenerat Med, Guangzhou Inst Biomed & Hlth, Key Lab Regenerat Biol, Guangzhou, Peoples R China

[4] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangdong Prov Key Lab Biocomp, Guangzhou, Peoples R China

来源：

STEM CELL RESEARCH | 2021年 / 51卷

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1016/j.scr.2021.102161

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Dickkopf1 (DKK1) is a secreted inhibitor for the Wnt signalling, which is involved in cell proliferation, tissue regeneration and embryonic development. Using CRISPR/Cas9 editing, we established a homozygous mutant DKK1 human embryonic stem cell line (WAe001-A-21). It has a 41 bp deletion in exon 2 of DKK1, leading to its coding frame shift. The WAe001-A-21 cell line maintains a normal karyotype, pluripotency markers, typical stem cell morphology and the ability to differentiate into three germ layers.

引用

页数：5

共 5 条

[1] Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction [J].