A novel bacteriocin with a broad inhibitory spectrum produced by Lactobacillus sake C2, isolated from traditional Chinese fermented cabbage

被引:69
作者
Gao, Yurong [1 ]
Jia, Shiru [1 ]
Gao, Qiang [1 ]
Tan, Zhilei [1 ]
机构
[1] Tianjin Univ Sci & Technol, Sch Biol Engn, Tianjin Key Lab Ind Microbiol, TEDA, Tianjin 300457, Peoples R China
关键词
Lactobacillus sakei; Bacteriocin; 16S rDNA sequence; Molecular weight; Traditional Chinese fermented cabbage; LACTIC-ACID BACTERIA; ENTEROCOCCUS-FAECIUM; SEQUENCE ALIGNMENT; BIOSYNTHESIS; PURIFICATION; PRESERVATION; GENETICS; MEAT; SOIL;
D O I
10.1016/j.foodcont.2009.04.003
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
By excluding the effects of organic acids and hydrogen peroxide, strain C2 producing a bacteriocin strongly inhibited Staphylococcus aureus ATCC 63589 and Escherichia coli ATCC 25922 was selected from 300 lactic acid bacteria isolated from traditional Chinese fermented cabbage. Strain C2 was identified as Lactobacillus sakei by phenotypical and physiological tests and 165 rDNA identification. This bacteriocin, which was designated sakacin C2, was purified by cold ethanol and Sephadex G50 column chromatography for further studies. The molecular weight of sakacin C2 was 5.5 kDa by Tris-Tricine SDS-PAGE, which was the largest among all known sakacins. Sakacin C2 exhibited a wide range of antimicrobial activity. strong heat stability ( 15 min at 121 degrees C) and pH stability (pH 3.0-8.0). Sakacin C2 was sensitive to protease but insensitive to lipase, alpha-amylase and beta-amylase. These characters suggested that sakacin C2 was a novel Class 11 bacteriocin with a broad inhibitory spectrum and might broaden the application range of bacteriocins from LAB in the food industry. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:76 / 81
页数:6
相关论文
共 33 条
  • [1] DETECTION AND CHARACTERIZATION OF A BACTERIOCIN PRODUCED BY LACTOBACILLUS-PLANTARUM C19
    ATRIH, A
    REKHIF, N
    MILLIERE, JB
    LEFEBVRE, G
    [J]. CANADIAN JOURNAL OF MICROBIOLOGY, 1993, 39 (12) : 1173 - 1179
  • [2] Isolation and identification of lactic acid bacteria from soil using an enrichment procedure
    Chen, YS
    Yanagida, F
    Shinohara, T
    [J]. LETTERS IN APPLIED MICROBIOLOGY, 2005, 40 (03) : 195 - 200
  • [3] Bacteriocins: safe, natural antimicrobials for food preservation
    Cleveland, J
    Montville, TJ
    Nes, IF
    Chikindas, ML
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2001, 71 (01) : 1 - 20
  • [4] Bacteriocin production by Lactobacillus pentosus B96 can be expressed as a function of temperature and NaCl concentration
    Delgado, A
    Brito, D
    Peres, C
    Noé-Arroyo, F
    Garrido-Fernández, A
    [J]. FOOD MICROBIOLOGY, 2005, 22 (06) : 521 - 528
  • [5] Characterization of anti-Listeria monocytogenes bacteriocins from Enterococcus faecalis, Enterococcus faecium, and Lactococcus lactis strains isolated from raib, a Moroccan traditional fermented milk
    Elotmani, F
    Revol-Junelles, AM
    Assobhei, O
    Millière, JB
    [J]. CURRENT MICROBIOLOGY, 2002, 44 (01) : 10 - 17
  • [6] Ennahar S, 2000, FEMS MICROBIOL REV, V24, P85, DOI 10.1016/S0168-6445(99)00031-5
  • [7] Production and characterization of enterocin 900, a bacteriocin produced by Enterococcus faecium BFE 900 from black olives
    Franz, CMAP
    Schillinger, U
    Holzapfel, WH
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1996, 29 (2-3) : 255 - 270
  • [8] Characterization of bacteriocin-like inhibitory substances (BLIS) from lactic acid bacteria isolated from traditional Azerbaijani cheeses
    Gulahmadov, Saib Gurban oglu
    Batdorj, Batjargal
    Dalgalarrondo, Michele
    Chobert, Jean-Marc
    Kuliev, Akif Alekper oglu
    Haertle, Thomas
    [J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2006, 224 (02) : 229 - 235
  • [9] LACTIC-ACID BACTERIA IN MEAT FERMENTATION
    HAMMES, WP
    BANTLEON, A
    MIN, S
    [J]. FEMS MICROBIOLOGY LETTERS, 1990, 87 (1-2) : 165 - 173
  • [10] PURIFICATION AND AMINO-ACID-SEQUENCE OF SAKACIN-A, A BACTERIOCIN FROM LACTOBACILLUS-SAKE LB706
    HOLCK, A
    AXELSSON, L
    BIRKELAND, SE
    AUKRUST, T
    BLOM, H
    [J]. JOURNAL OF GENERAL MICROBIOLOGY, 1992, 138 : 2715 - 2720