In vitro activity, extraction, separation and structure elucidation of antibiotic produced by Streptomyces anulatus NEAE-94 active against multidrug-resistant Staphylococcus aureus

被引：42

作者：

El-Naggar, Noura El-Ahmady ^{[1
]}

El-Bindary, Ashraf Abdal-Aziz ^{[2
]}

Abdel-Mogib, Mamdouh ^{[3
]}

Nour, Noura Salah ^{[1
]}

机构：

[1] City Sci Res & Technol Applicat, Genet Engn & Biotechnol Res Inst, Dept Bioproc Dev, Alexandria, Egypt

[2] Damietta Univ, Dept Chem, Fac Sci, Dumyat, Egypt

[3] Mansoura Univ, Dept Chem, Fac Sci, Mansoura, Egypt

来源：

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT | 2017年 / 31卷 / 02期

关键词：

Streptomyces anulatus; antibiotic; H-1-NMR; FT-IR; GC-MS analysis; multidrug-resistant Staphylococcus aureus; GRAM-POSITIVE BACTERIA; FATTY-ACIDS; ANTIMICROBIAL ACTIVITY; METABOLITES; DIVERSITY; MECHANISMS; GROWTH; SKIN;

D O I：

10.1080/13102818.2016.1276412

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

During the screening programme for antibiotic produced by actinomycetes, an antibiotic was isolated from the fermentation broth of Streptomyces anulatus NEAE-94. The ethyl acetate extract of S. anulatus NEAE-94 showed high biological activity against Staphylococcus aureus NRRL B-313, multidrug-resistant S. aureus and Bacillus subtilis NRRL B-543. Thin-layer chromatography of the ethyl acetate extract showed one yellowish orange spot with an Rf value of 0.83. The structural elucidation of the isolated compounds was carried out using ultraviolet-visible mass spectrometry (MS), Fourier transform-infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (H-1-NMR) and gas chromatography-mass spectrometry (GC-MS) analyses. Characterization of the ethyl acetate extract revealed the presence of 22 compounds, including an unsaturated fatty acid, saturated fatty acids, fatty acid esters, alkanes, alkenes and a triterpene. The antimicrobial efficacy of S. anulatus NEAE-94 might be due to the synergistic effects of the identified compounds.

引用

页码：418 / 430

页数：13

共 60 条

[1]

Abou-elela G., 2009, The Scientific World Journal, V7, P872

[2]

Akpuaka A., 2013, NY SCI J, V6, P119

[3] A non-polyene antifungal antibiotic from Streptomyces albidoflavus PU 23 [J].

Augustine, SK ;

Bhavsar, SP ;

Kapadnis, BP .

JOURNAL OF BIOSCIENCES, 2005, 30 (02) :201-211

[4] Chemical composition, cytotoxicity effect and antimicrobial activity of Ceratonia siliqua essential oil with preservative effects against Listeria inoculated in minced beef meat [J].

Ben Hsouna, Anis ;

Trigui, Mohamed ;

Ben Mansour, Riadh ;

Jarraya, Raoudha Mezghani ;

Damak, Mohamed ;

Jaoua, Samir .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2011, 148 (01) :66-72

[5] Bioactive microbial metabolites -: A personal view [J].

Bérdy, J .

JOURNAL OF ANTIBIOTICS, 2005, 58 (01) :1-26

[6] New agents for Gram-positive bacteria [J].

Bhavnani, SM ;

Ballow, CH .

CURRENT OPINION IN MICROBIOLOGY, 2000, 3 (05) :528-534

[7] SENSITIVITY OF STAPHYLOCOCCUS-AUREUS TO UNSATURATED FATTY-ACIDS [J].

BUTCHER, GW ;

KING, G ;

DYKE, KGH .

JOURNAL OF GENERAL MICROBIOLOGY, 1976, 94 (JUN) :290-296

[8] CORRELATION OF CAROTENOID PRODUCTION, DECREASED MEMBRANE FLUIDITY, AND RESISTANCE TO OLEIC-ACID KILLING IN STAPHYLOCOCCUS-AUREUS 18Z [J].

CHAMBERLAIN, NR ;

MEHRTENS, BG ;

XIONG, Z ;

KAPRAL, FA ;

BOARDMAN, JL ;

REARICK, JI .

INFECTION AND IMMUNITY, 1991, 59 (12) :4332-4337

[9] Waves of resistance: Staphylococcus aureus in the antibiotic era [J].

Chambers, Henry F. ;

DeLeo, Frank R. .

NATURE REVIEWS MICROBIOLOGY, 2009, 7 (09) :629-641

[10] New lactone-containing metabolites from a marine-derived bacterium of the genus Streptomyces [J].

Cho, KW ;

Lee, HS ;

Rho, JR ;

Kim, TS ;

Mo, SJ ;

Shin, J .

JOURNAL OF NATURAL PRODUCTS, 2001, 64 (05) :664-667

← 1 2 3 4 5 6 →