Reference Standardization for Mass Spectrometry and High-resolution Metabolomics Applications to Exposome Research

被引:176
作者
Go, Young-Mi [1 ]
Walker, Douglas I. [1 ,2 ]
Liang, Yongliang [1 ]
Uppal, Karan [1 ]
Soltow, Quinlyn A. [1 ]
ViLinh Tran [1 ]
Strobel, Frederick [3 ]
Quyyumi, Arshed A. [4 ]
Ziegler, Thomas R. [5 ]
Pennell, Kurt D. [2 ]
Miller, Gary W. [6 ]
Jones, Dean P. [1 ]
机构
[1] Emory Univ, Dept Med, Div Pulm Allergy & Crit Care Med, Atlanta, GA 30322 USA
[2] Tufts Univ, Dept Civil & Environm Engn, Medford, MA 02155 USA
[3] Dept Chem, Atlanta, GA 30322 USA
[4] Emory Univ, Dept Med, Cardiol, Atlanta, GA 30322 USA
[5] Emory Univ, Dept Med, Endocrinol Metab & Lipids, Atlanta, GA 30322 USA
[6] Emory Univ, Dept Environm Hlth Sci, Atlanta, GA 30322 USA
基金
美国国家卫生研究院;
关键词
amino acids; metabolomics; analytical chemistry; environment; mass spectrometry; LIQUID-CHROMATOGRAPHY; LARGE-SCALE; GAS-CHROMATOGRAPHY; HUMAN PLASMA; UPLC-MS; PROFILES; SERUM; PROTEINS; DATABASE; DIETARY;
D O I
10.1093/toxsci/kfv198
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
The exposome is the cumulative measure of environmental influences and associated biological responses throughout the lifespan, including exposures from the environment, diet, behavior, and endogenous processes. A major challenge for exposome research lies in the development of robust and affordable analytic procedures to measure the broad range of exposures and associated biologic impacts occurring over a lifetime. Biomonitoring is an established approach to evaluate internal body burden of environmental exposures, but use of biomonitoring for exposome research is often limited by the high costs associated with quantification of individual chemicals. High-resolution metabolomics (HRM) uses ultra-high resolution mass spectrometry with minimal sample preparation to support high-throughput relative quantification of thousands of environmental, dietary, and microbial chemicals. HRM also measures metabolites in most endogenous metabolic pathways, thereby providing simultaneous measurement of biologic responses to environmental exposures. The present research examined quantification strategies to enhance the usefulness of HRM data for cumulative exposome research. The results provide a simple reference standardization protocol in which individual chemical concentrations in unknown samples are estimated by comparison to a concurrently analyzed, pooled reference sample with known chemical concentrations. The approach was tested using blinded analyses of amino acids in human samples and was found to be comparable to independent laboratory results based on surrogate standardization or internal standardization. Quantification was reproducible over a 13-month period and extrapolated to thousands of chemicals. The results show that reference standardization protocol provides an effective strategy that will enhance data collection for cumulative exposome research. In principle, the approach can be extended to other types of mass spectrometry and other analytical methods.
引用
收藏
页码:531 / 543
页数:13
相关论文
共 47 条
[1]   Large-scale human metabolomics studies: A strategy for data (pre-) processing and validation [J].
Bijlsma, S ;
Bobeldijk, L ;
Verheij, ER ;
Ramaker, R ;
Kochhar, S ;
Macdonald, IA ;
van Ommen, B ;
Smilde, AK .
ANALYTICAL CHEMISTRY, 2006, 78 (02) :567-574
[2]   Isotopic labeling-assisted metabolomics using LC-MS [J].
Bueschl, C. ;
Krska, R. ;
Kluger, B. ;
Schuhmacher, R. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2013, 405 (01) :27-33
[3]   Quantitative profiling of proteins in complex mixtures using liquid chromatography and mass spectrometry [J].
Chelius, D ;
Bondarenko, PV .
JOURNAL OF PROTEOME RESEARCH, 2002, 1 (04) :317-323
[4]   Untargeted Plasma Metabolite Profiling Reveals the Broad Systemic Consequences of Xanthine Oxidoreductase Inactivation in Mice [J].
Chen, Qiuying ;
Park, Hyeong-Cheon ;
Goligorsky, Michael S. ;
Chander, Praveen ;
Fischer, Steven M. ;
Gross, Steven S. .
PLOS ONE, 2012, 7 (06)
[5]  
Cribbs SK, 2014, AIDS RES HUM RETROV, V30, P579, DOI [10.1089/aid.2013.0198, 10.1089/AID.2013.0198]
[6]   Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry [J].
Dunn, Warwick B. ;
Broadhurst, David ;
Begley, Paul ;
Zelena, Eva ;
Francis-McIntyre, Sue ;
Anderson, Nadine ;
Brown, Marie ;
Knowles, Joshau D. ;
Halsall, Antony ;
Haselden, John N. ;
Nicholls, Andrew W. ;
Wilson, Ian D. ;
Kell, Douglas B. ;
Goodacre, Royston .
NATURE PROTOCOLS, 2011, 6 (07) :1060-1083
[7]   Children with severe asthma have unique oxidative stress-associated metabolomic profiles [J].
Fitzpatrick, Anne M. ;
Park, Youngja ;
Brown, Lou Ann S. ;
Jones, Dean P. .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2014, 133 (01) :258-261
[8]   Plasma Metabolomics in Human Pulmonary Tuberculosis Disease: A Pilot Study [J].
Frediani, Jennifer K. ;
Jones, Dean P. ;
Tukvadze, Nestan ;
Uppal, Karan ;
Sanikidze, Eka ;
Kipiani, Maia ;
Tran, ViLinh T. ;
Hebbar, Gautam ;
Walker, Douglas I. ;
Kempker, Russell R. ;
Kurani, Shaheen S. ;
Colas, Romain A. ;
Dalli, Jesmond ;
Tangpricha, Vin ;
Serhan, Charles N. ;
Blumberg, Henry M. ;
Ziegler, Thomas R. .
PLOS ONE, 2014, 9 (10)
[9]  
Go Young-Mi, 2014, Methods Mol Biol, V1198, P43, DOI 10.1007/978-1-4939-1258-2_4
[10]   Metabolome-wide association study of phenylalanine in plasma of common marmosets [J].
Go, Young-Mi ;
Walker, Douglas I. ;
Soltow, Quinlyn A. ;
Uppal, Karan ;
Wachtman, Lynn M. ;
Strobel, Fredrick H. ;
Pennell, Kurt ;
Promislow, Daniel E. L. ;
Jones, Dean P. .
AMINO ACIDS, 2015, 47 (03) :589-601