Drosophila mojoless, a retroposed GSK-3, has functionally diverged to acquire an essential role in male fertility

被引:26
作者
Kalamegham, Rasika [1 ]
Sturgill, David
Siegfried, Esther
Oliver, Brian
机构
[1] NIDDKD, NIH, Lab Cellular & Dev Biol, Bethesda, MD 20892 USA
[2] Penn State Univ, Dept Biochem Mol Biol & Microbiol, University Pk, PA 16802 USA
关键词
germ cell; male sterile; gene duplication; GSK-3; adaptive evolution; retroposition; male germ line;
D O I
10.1093/molbev/msl201
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Retroposition is increasingly recognized as an important mechanism for the acquisition of new genes. We show that a glycogen synthase kinase-3 gene, shaggy (sgg), retroposed at least 50 MYA in the Drosophila genus to generate a new gene, mojoless (mjl). We have extensively analyzed the function of mjl and examined its functional divergence from the parental gene sgg in Drosophila melanogaster. Unlike Sgg, which is expressed in many tissues of both sexes, Mjl is expressed specifically in the male germ line, where it is required for male germ line survival. Our analysis indicates that mjl has acquired a specific function in the maintenance of male germ line viability. However, it has not completely lost its ancestral biochemical function and can partially compensate for loss of the parental gene sgg when ectopically expressed in somatic cells. We postulate that mjl has undergone functional diversification and is now under stabilizing selection in the Drosophila genus.
引用
收藏
页码:732 / 742
页数:11
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