Implications of the epidermal growth factor on burn skin wound repair. An in vitro and in vivo study

An injury healing process after tissue damage comprises of a profoundly directed succession of events. This paper outlines the role of EGF in tissue recuperating and tissue repair, and presents its clinical utility as restorative agent for different injury. The enhanced dermal regeneration by the use of EGF observed in open injuries may improve the wound healing and scar tissue. The cell viability was found to be 123%, 132% and 144% higher than those of the control group at 0.1, 0.5 and 1.0 micrometer of rLMP-EGF. The rEGF demonstrated 123%, 135% and 146% increments in cell expansion at 0.1, 0.5 and 1.0 mu M, separately, thereby demonstrating that the biological activity of rLMP-EGF and rEGF was comparable. The conjugation of low molecular protamine did not adversely affect EGF activity. The rEGF treatment (10 mg/mL) reduced the injury range, when compared with the results found in the control group. An appreciable improvement was noticed on the fourth day. The injured tissue was also assessed histologically. Application of rLMP-EGF or rEGF demonstrated maximum re-epithelisation while no recovery of the epidermis was observed in injuries treated with a vehicle.