Probing key targets in insulin signaling and adipogenesis using a methanolic extract of Costus pictus and its bioactive molecule, methyl tetracosanoate

被引：23

作者：

Shilpa, Kusampudi ^{[1
]}

Sangeetha, Kadapakkam Nandabalan ^{[1
]}

Muthusamy, Velusamy Shanmuganathan ^{[1
]}

Sujatha, Sundaresan ^{[1
]}

Lakshmi, Baddireddi Subadhra ^{[1
]}

机构：

[1] Anna Univ, Ctr Biotechnol, Madras 600025, Tamil Nadu, India

来源：

BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 12期

关键词：

Adipogenesis; Costus pictus; Glucose uptake; Methyl tetracosanoate; Type; 2; diabetes; OBESITY; CONVERSION; MUSCLE;

D O I：

10.1007/s10529-009-0105-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A methanolic extract of Costus pictus (CPME) showed optimum anti-diabetic activity at 100 ng/ml. Bioactivity-guided purification of CPME led to the isolation of methyl tetracosanoate (MT) which showed an optimum glucose uptake at 1 ng/ml. CPME at 10 mu g/ml inhibited adipogenesis whereas fully differentiated adipocytes exhibited a 3-fold increase in lipid accumulation compared to pre-adipocytes. Gene and protein expression of key targets in insulin signaling and adipogenesis pathway revealed that CPME exhibited anti-diabetic activity along with anti-adipogenic activity whereas MT demonstrated only anti-diabetic activity.

引用

页码：1837 / 1841

页数：5

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