Identification and characterization of a novel whitefly-transmitted member of the family Potyviridae isolated from cucurbits in Florida

被引:58
作者
Adkins, Scott [1 ]
Webb, Susan E.
Achor, Diann
Roberts, Pamela D.
Baker, Carlye A.
机构
[1] Dept Agr Agr Res Serv, Ft Pierce, FL 34945 USA
[2] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA
[3] Univ Florida, Ctr Citrus Res & Educ, Lake Alfred, FL 33850 USA
[4] Univ Florida, Dept Plant Pathol, SW Florida Res & Educ Ctr, Immokalee, FL USA
[5] Florida Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL 32614 USA
基金
英国工程与自然科学研究理事会;
关键词
D O I
10.1094/PHYTO-97-2-0145
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A novel whitefly-transmitted member of the family Potyviridae was isolated from a squash plant (Cucurbita pepo) with vein yellowing symptoms in Florida. The virus, for which the name Squash vein yellowing virus (SqVYV) is proposed, has flexuous rod-shaped particles of 840 nm in length. The experimental host range was limited to species in the family Cucurbitaceae, with the most dramatic symptoms observed in squash and watermelon, but excluded all tested species in the families Amaranthaceae, Apocynaceae, Asteraceae, Chenopodiaceac, Fabaceae, Malvaceae, and Solanaceae. The virus was transmitted by whiteflies (Bemisia tobaci) but was not transmitted by aphids (Myzus persicae). Infection by SqVYV induced inclusion bodies visible by electron and light microscopy that were characteristic of members of the family Potyviridae. Comparison of the SqVYV coat protein gene and protein sequences with those of recognized members of the family Potyviridae indicate that it is a novel member of the genus Ipomovirus. A limited survey revealed that SqVYV also was present in watermelon plants suffering from a vine decline and fruit rot recently observed in Florida and was sufficient to induce these symptoms in greenhouse-grown watermelon, suggesting that SqVYV is the likely cause of this disease.
引用
收藏
页码:145 / 154
页数:10
相关论文
共 56 条
[1]   Molecular criteria for genus and species discrimination within the family Potyviridae [J].
Adams, MJ ;
Antoniw, JF ;
Fauquet, CM .
ARCHIVES OF VIROLOGY, 2005, 150 (03) :459-479
[2]  
Al-Musa A. M., 1985, Plant Disease, V69, P361
[3]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[4]  
[Anonymous], ATLAS INSECT PLANT V
[5]  
[Anonymous], J ULTRASTRUCT RES
[6]  
[Anonymous], 2001, Anal Biochem
[7]  
Berger PH, 2005, VIRUS TAXONOMY, P819
[8]   OCCURRENCE OF BEAN GOLDEN MOSAIC-VIRUS IN FLORIDA [J].
BLAIR, MW ;
BASSETT, MJ ;
ABOUZID, AM ;
HIEBERT, E ;
POLSTON, JE ;
MCMILLAN, RT ;
GRAVES, W ;
LAMBERTS, M .
PLANT DISEASE, 1995, 79 (05) :529-533
[9]  
BLANCARD D, 1994, COLOUR ATLAS CUCURBI, P238
[10]   Etiology of leaf chlorosis and deterioration of the fruit interior of watermelon plants [J].
Boubourakas, IN ;
Hatziloukas, E ;
Antignus, Y ;
Katis, NI .
JOURNAL OF PHYTOPATHOLOGY, 2004, 152 (10) :580-588