Ultrahigh-resolution backbone structure of perdeuterated protein GB1 using residual dipolar couplings from two alignment media

被引：23
作者：
Bouvignies, Guillaume
Meier, Sebastian
Grzesiek, Stephan
Blackledge, Martin
机构：
[1] Univ Basel, Biozentrum, CH-4056 Basel, Switzerland
[2] Univ Grenoble 1, CNRS, CEA, UMR 5075,Inst Biol Struct Jean Pierre Ebel, F-38027 Grenoble, France
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 48期
关键词：
NMR spectroscopy; protein dynamics; protein structures; residual dipolar couplings;
D O I：
10.1002/anie.200603627
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Chemical Equation Presented) NMR dipolar couplings can be measured for weakly aligned proteins and are sensitive probes of their structure and dynamics. The combination of fixed geometry and 1H-1H couplings alone from two alignment media provides access to ultrahigh-resolution structures. The apparent precision, as measured by independent cross-validation and structure comparison, is comparable to that available from high-resolution X-ray crystallography. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：8166 / 8169
页数：4
相关论文
共 20 条
[1]   Direct structure determination using residual dipolar couplings:: Reaction-site conformation of methionine sulfoxide reductase in solution [J].
Béraud, S ;
Bersch, B ;
Brutscher, B ;
Gans, P ;
Barras, F ;
Blackledge, M .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (46) :13709-13715
[2]   Anisotropic small amplitude peptide plane dynamics in proteins from residual dipolar couplings [J].
Bernadó, P ;
Blackledge, M .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (15) :4907-4920
[3]   Recent progress in the study of biomolecular structure and dynamics in solution from residual dipolar couplings [J].
Blackledge, M .
PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 2005, 46 (01) :23-61
[4]   Identification of slow correlated motions in proteins using residual dipolar and hydrogen-bond scalar couplings [J].
Bouvignies, G ;
Bernadó, P ;
Meier, S ;
Cho, K ;
Grzesiek, S ;
Brüschweiler, R ;
Blackledge, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (39) :13885-13890
[5]   Protein backbone dynamics from N-HN dipolar couplings in partially aligned systems:: a comparison of motional models in the presence of structural noise [J].
Bouvignies, G ;
Bernadó, P ;
Blackledge, M .
JOURNAL OF MAGNETIC RESONANCE, 2005, 173 (02) :328-338
[6]  
BRUSCHWEILER R, 1994, J AM CHEM SOC, V116, P8426
[7]   Protein side-chain rotamers from dipolar couplings in a liquid crystalline phase [J].
Chou, JJ ;
Bax, A .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (16) :3844-3845
[8]  
Clore GM, 1997, NAT STRUCT BIOL, V4, P849
[9]   Protein structure determination using molecular fragment replacement and NMR dipolar couplings [J].
Delaglio, F ;
Kontaxis, G ;
Bax, A .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (09) :2142-2143
[10]   THE 3RD IGG-BINDING DOMAIN FROM STREPTOCOCCAL PROTEIN-G - AN ANALYSIS BY X-RAY CRYSTALLOGRAPHY OF THE STRUCTURE ALONE AND IN A COMPLEX WITH FAB [J].
DERRICK, JP ;
WIGLEY, DB .
JOURNAL OF MOLECULAR BIOLOGY, 1994, 243 (05) :906-918
12