Comparative PCR analysis of toxic Nodularia spumigena and non-toxic Nodularia harveyana (Nostocales, Cyanobacteria) with respect to the nodularin synthetase gene cluster

被引：2
作者：
Krueger, Thomas [1 ]
Oelmueller, Ralf [2 ]
Luckas, Bernd [1 ]
机构：
[1] Univ Jena, Inst Nutr, D-07743 Jena, Germany
[2] Univ Jena, Inst Gen Bot, D-07743 Jena, Germany
来源：
EUROPEAN JOURNAL OF PHYCOLOGY | 2009年 / 44卷 / 03期
关键词：
Baltic Sea; hepatotoxins; Nodularia; nodularin; pentapeptide; peptide synthetase; BALTIC SEA; BENTHIC CYANOBACTERIA; MICROCYSTIN; EVOLUTION; HEPATOTOXINS;
D O I：
10.1080/09670260802588434
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
A comparative PCR analysis of the nodularin synthetase gene cluster (nda) of two closely related Nodularia strains from the Baltic Sea was performed. Unlike the nodularin-producing Nodularia spumigena Huebel 1988/306, all 11 tested nda sequences were absent in the non-toxic Nodularia harveyana Huebel 1983/300. Our results suggest that the benthic N. harveyana strain lacks the entire 48-kb nodularin synthetase gene cluster. We describe new primers and the requisite experimental conditions for screening different nda sequences.
引用
收藏
页码：291 / 295
页数：5
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