Molecular characterizations of chloramphenicol- and oxytetracycline-resistant bacteria and resistance genes in mariculture waters of China

被引：31
作者：
Dang, Hongyue [1 ]
Zhao, Jingyi [1 ]
Song, Linsheng [2 ]
Chen, Mingna [2 ]
Chang, Yaqing [3 ]
机构：
[1] China Univ Petr E China, Ctr Bioengn & Biotechnol, Qingdao 266555, Peoples R China
[2] Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China
[3] Dalian Fisheries Univ, Key Lab Mariculture & Biotechnol, Dalian 116023, Peoples R China
来源：
MARINE POLLUTION BULLETIN | 2009年 / 58卷 / 07期
基金：
中国国家自然科学基金;
关键词：
Multidrug resistance; Resistance genes; Marine vibrios; Multiplex PCR; Mariculture; POLYMERASE-CHAIN-REACTION; VIBRIO-SPLENDIDUS; ANTIBIOTIC-RESISTANCE; SCOPHTHALMUS-MAXIMUS; AQUATIC ENVIRONMENTS; EDWARDSIELLA-TARDA; CRASSOSTREA-GIGAS; MARINE WATERS; SEA-CUCUMBER; JIAOZHOU BAY;
D O I：
10.1016/j.marpolbul.2009.02.016
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
In order to gain an understanding of the diversity and distribution of antimicrobial-resistant bacteria and their resistance genes in maricultural environments, multidrug-resistant bacteria were screened for the rearing waters from a mariculture farm of China. Both abalone Haliotis discus hannai and turbot Scophthalmus maximus rearing waters were populated with abundant chloramphenicol-resistant bacteria. These bacteria were also multidrug resistant, with Vibrio splendidus and Vibrio tasmaniensis being the most predominant species. The chloramphenicol-resistance gene cat II, cat IV or floR could be detected in most of the multidrug-resistant isolates, and the oxytetracycline-resistance gene tet(B), tet(D), tet(E) or tet(M) could also be detected for most of the isolates. Coexistence of chloramphenicol- and oxytetracycline-resistance genes partially explains the molecular mechanism of multidrug resistance in the studied maricultural environments. Comparative studies with different antimicrobial agents as the starting isolation reagents may help detect a wider diversity of the antimicrobial-resistant bacteria and their resistance genes. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码：987 / 994
页数：8
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