Mitigation Effect of Proanthocyanidin on Secondary Heart Injury in Rats Caused by Mechanical Trauma

被引:12
作者
Ma, Shuo [1 ]
Chen, Chong [1 ]
Cao, Tingting [1 ]
Bi, Yue [1 ]
Zhou, Jicheng [1 ]
Li, Xintao [1 ]
Yu, Deqin [1 ]
Li, Shuzhuang [1 ]
机构
[1] Dalian Med Univ, Dept Physiol, Dalian 116044, Peoples R China
来源
SCIENTIFIC REPORTS | 2017年 / 7卷
关键词
ACUTE MYOCARDIAL-INFARCTION; GRAPE SEED PROANTHOCYANIDIN; INDUCED CYTOKINE PRODUCTION; NECROSIS-FACTOR-ALPHA; CARDIOMYOCYTE APOPTOSIS; CARDIAC DYSFUNCTION; FREE-RADICALS; CELL LINE; PROCYANIDINS; REPERFUSION;
D O I
10.1038/srep44623
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Multiple organ dysfunctional syndrome secondary to mechanical trauma (MT) has attracted considerable research attention. The heart is one of the most important organs of the body, and secondary cardiac insufficiency caused by MT seriously affects the quality of life. This study aims to investigate whether proanthocyanidin can alleviate myocardial injury and improve heart function in the process of MT leading to secondary cardiac insufficiency. Noble-Collip drum wasused to prepare MT model in rats. And myocardial apoptosis index was calculated after TUNEL staining. Ventricular intubation was employed to detect heart function. Changes in myocardial ultrastructure were observed using an electron microscope. ELISA was used to detect the content of TNF-alpha and reactive oxygen species generated from monocytes and cardiomyocytes. The changes in Ca2+ concentration in cardiomyocyte were observed by confocal microscope. Compared with trauma group, the administration group had a decreased apoptosis index of cardiomyocytes, and increased +/- dp/dtmax. Meanwhile, proanthocyanidin can inhibit monocytes' TNF-alpha production, and reduce plasma TNF-alpha concentration. Moreover, proanthocyanidin can attenuate the excessive oxidative stress reaction of cardiomyocyte, and inhibit calcium overload in cardiomyocytes. In conclusion, proanthocyanidin can effectively ease myocardial damage and improve cardiac function, through anti-inflammatory and antioxidant effects in secondary cardiac insufficiency caused by MT.
引用
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页数:12
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