Chai-Qin-Cheng-Qi Decoction and Carbachol Improve Intestinal Motility by Regulating Protein Kinase C-Mediated Ca2+ Release in Colonic Smooth Muscle Cells in Rats with Acute Necrotising Pancreatitis

被引：16

作者：

Zhang, Chen-Long ^{[1
]}

Lin, Zi-Qi ^{[1
]}

Luo, Rui-Jie ^{[1
]}

Zhang, Xiao-Xin ^{[1
]}

Guo, Jia ^{[1
]}

Wu, Wei ^{[1
]}

Shi, Na ^{[1
]}

Deng, Li-Hui ^{[1
]}

Chen, Wei-Wei ^{[1
]}

Zhang, Xiao-Ying ^{[1
,2
]}

Bharucha, Shameena ^{[2
]}

Huang, Wei ^{[1
,2
]}

Sutton, Robert ^{[2
]}

Windsor, John A. ^{[3
]}

Xue, Ping ^{[1
]}

Xia, Qing ^{[1
]}

机构：

[1] Sichuan Univ, West China Hosp, Dept Integrated Tradit Chinese & Western Med, Sichuan Prov Pancreatitis Ctr, Chengdu, Peoples R China

[2] Univ Liverpool, Royal Liverpool Univ Hosp NHS Trust, NIHR Liverpool Pancreas Biomed Res Unit, Liverpool, Merseyside, England

[3] Univ Auckland, Dept Surg, Auckland, New Zealand

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2017年 / 2017卷

关键词：

DETERMINANT-BASED CLASSIFICATION; ABDOMINAL COMPARTMENT SYNDROME; ORGAN FAILURE; MESENTERIC LYMPH; DYSFUNCTION; GUT; RECEPTORS; SEVERITY; ARGININE; METAANALYSIS;

D O I：

10.1155/2017/5864945

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Chai-Qin-Cheng-Qi decoction (CQCQD) improves intestinal motility in acute pancreatitis (AP), but the mechanism(s) require elucidation. We investigated the effects of CQCQD and carbachol, a prokinetic agent, on colonic smooth muscle cells (SMCs) in L-arginine-induced necrotising AP model in rats. In treatment groups, intragastric CQCQD (20 g/kg, 2 hourly x 3 doses) or intraperitoneal carbachol (60 mu g/kg) was given 24 hours after induction of AP. Both CQCQD and carbachol decreased the severity of pancreatic and colonic histopathology (all P < 0.05). Both CQCQD and carbachol reduced serum intestinal fatty acid binding protein, vasoactive intestinal peptide, and substance P and increased motility levels. CQCQD upregulated SMC phospholipase Cbeta 1 (PLC-beta 1) mRNAand PLCprotein (both P< 0.05), while both treatments upregulated protein kinaseC-alpha (PKC-alpha) mRNA and PKC protein and downregulated adenylate cyclase (AC) mRNAand protein compared with no treatment (all P < 0.05). Neither treatment significantly altered L-arginine-induced PKC-beta 1 and PKC-epsilon mRNA reduction. Both treatments significantly increased fluorescence intensity of SMC intracellular calcium concentration [Ca2+] i (3563.5 and 3046.9 versus 1086.9, both P< 0.01). These data suggest CQCQD and carbachol improve intestinalmotility in AP by increasing [Ca2+] i in colonic SMCs via upregulating PLC, PKC and downregulating AC.

引用

页数：12

共 51 条

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