High-speed atomic force microscope based on an astigmatic detection system

被引：15

作者：

Liao, H-S ^{[1
]}

Chen, Y-H ^{[1
]}

Ding, R-F ^{[1
,2
]}

Huang, H-F ^{[1
,2
]}

Wang, W-M ^{[1
,2
]}

Hwu, E-T ^{[1
]}

Huang, K-Y ^{[2
]}

Chang, C-S ^{[1
]}

Hwang, I-S ^{[1
]}

机构：

[1] Acad Sinica, Inst Phys, Taipei 11529, Taiwan

[2] Natl Taiwan Univ, Dept Mech Engn, Taipei 10617, Taiwan

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 2014年 / 85卷 / 10期

关键词：

CANTILEVERS; SURFACE; PROBE;

D O I：

10.1063/1.4898019

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

High-speed atomic force microscopy (HS-AFM) enables visualizing dynamic behaviors of biological molecules under physiological conditions at a temporal resolution of 1s or shorter. A small cantilever with a high resonance frequency is crucial in increasing the scan speed. However, detecting mechanical resonances of small cantilevers is technically challenging. In this study, we constructed an atomic force microscope using a digital versatile disc (DVD) pickup head to detect cantilever deflections. In addition, a flexure-guided scanner and a sinusoidal scan method were implemented. In this work, we imaged a grating sample in air by using a regular cantilever and a small cantilever with a resonance frequency of 5.5 MHz. Poor tracking was seen at the scan rate of 50 line/s when a cantilever for regular AFM imaging was used. Using a small cantilever at the scan rate of 100 line/s revealed no significant degradation in the topographic images. The results indicate that a smaller cantilever can achieve a higher scan rate and superior force sensitivity. This work shows the potential for using a DVD pickup head in future HS-AFM technology. (C) 2014 AIP Publishing LLC.

引用

页数：7

共 30 条

[1] A high-speed atomic force microscope for studying biological macromolecules [J].