Climbing Fiber Projections in Relation to Zebrin Stripes in the Ventral Uvula in Pigeons

The cerebellum consists of sagittally oriented zones that are delineated by afferent input, Purkinje cell response properties, and the expression of molecular markers such as zebrin II (ZII). ZII is heterogeneously expressed in Purkinje cells such that there are parasagittal stripes of high expression (ZII+) interdigitated with stripes of little or no expression (ZII-). In pigeons, folium IXcd consists of seven pairs of ZII+/- stripes denoted P1+/- (medial) to P7+/- (lateral). In the present study we examined the climbing fiber input to the medial half of folium IXcd, the ventral uvula, which spans the medial two stripe pairs (P1+/- to P2+/-). Purkinje cells in the ventral uvula respond to patterns of optic flow resulting from self-motion through the environment along translational axes and their climbing fibers originate in the lateral half of the medial column in the inferior olive (mcIO). Using anterograde injections into this region of the mcIO, we found the following topographic relationship: climbing fibers from the caudal lateral mcIO were located in P1+ and medial P1- ZII stripes; climbing fibers from the rostral lateral mcIO were located in lateral P2+ and P2- ZII stripes, and climbing fibers from the middle lateral mcIO were located in lateral P1- and medial P2+ ZII stripes. These data complement our previous findings showing a topographic relationship between Purkinje cell responses to optic flow visual stimuli and ZII stripes. Taken together, we suggest that a ZII+/- stripe pair may represent a functional unit in the pigeon vestibulocerebellum. J. Comp. Neurol. 522:3629-3643, 2014. (c) 2014 Wiley Periodicals, Inc.