Subnanomolar detection of ochratoxin A using aptamer-attached silver nanoparticles and surface-enhanced Raman scattering

被引：33

作者：

Ganbold, Erdene-Ochir ^{[1
]}

Lee, Cheol Min ^{[2
]}

Cho, Eun-Min ^{[3
]}

Son, Sang Jun ^{[4
,5
]}

Kim, Sehun ^{[6
,7
]}

Joo, Sang-Woo ^{[1
]}

Yang, Sung Ik ^{[3
]}

机构：

[1] Soongsil Univ, Dept Chem, Seoul 156743, South Korea

[2] Hanyang Univ, Inst Environm & Ind Med, Seoul 133791, South Korea

[3] Kyung Hee Univ, Coll Environm & Appl Chem, Yongin 446701, South Korea

[4] Gachon Univ, Dept Chem, Songnam 461701, South Korea

[5] Gil Med Ctr, Korea & Gachon Med Res Inst, Inchon 405760, South Korea

[6] Korea Adv Inst Sci & Technol, Mol Level Interface Res Ctr, Taejon 305701, South Korea

[7] Korea Adv Inst Sci & Technol, Dept Chem, Taejon 305701, South Korea

来源：

ANALYTICAL METHODS | 2014年 / 6卷 / 11期

关键词：

UNMODIFIED GOLD NANOPARTICLES; SPECTROSCOPY; PLATFORM;

D O I：

10.1039/c4ay00440j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report a new approach for the detection of ochratoxin A (OTA) by introducing surface-enhanced Raman scattering (SERS). This method is based on different adsorption propensities of Raman reporter Cy5-tagged OTA aptamer. The OTA aptamer could be easily adsorbed onto the surface of silver nanoparticles (AgNPs) to give fairly strong SERS signals, whereas such bindings would be hampered in the presence of OTA. On the addition of OTA in the concentration range of 0.1-10 nM, the SERS signals appeared to decrease by similar to 40%, since OTA's aptamer could not adsorb onto the surface of AgNPs due to binding with OTA. We verified our results against a control experiment with warfarin, which did not affect the SERS signals. A nanomolar detection limit of OTA was achieved using the current SERS-based method.

引用

页码：3573 / 3577

页数：5

共 29 条

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