Stable isotope-labeled tracers for the investigation of fatty acid and triglyceride metabolism in humans in vivo

被引：37

作者：

Magkos, Faidon ^{[1
,2
]}

Mittendorfer, Bettina ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Div Geriatr & Nutr Sci, St Louis, MO 63110 USA

[2] Harokopio Univ, Dept Nutr & Dietet, Athens, Greece

来源：

CLINICAL LIPIDOLOGY | 2009年 / 4卷 / 02期

关键词：

adipose tissue; lipid metabolism; lipolysis; lipoprotein; liver; mass spectrometry; oxidation; LOW-DENSITY LIPOPROTEIN; HUMAN SKELETAL-MUSCLE; DE-NOVO LIPOGENESIS; SUBCUTANEOUS ADIPOSE-TISSUE; VLDL-TRIGLYCERIDE; PLASMA TRIGLYCERIDE; APOLIPOPROTEIN B-100; LIPID-METABOLISM; DIETARY-FAT; WHOLE-BODY;

D O I：

10.2217/CLP.09.9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Understanding lipid metabolism and its regulation requires information concerning the rates at which lipids are produced within the body, absorbed (dietary lipids) into the body, transported within the body, and utilized by various tissues. This article focuses on the use of stable isotope-labeled tracers for the quantitative evaluation of major pathways of fatty acid and triglyceride metabolism in humans in vivo. Adipose tissue lipolysis and free fatty acid appearance in plasma, fatty acid tissue uptake and oxidation, and hepatic VLDL triglyceride secretion are among the metabolic pathways that can be studied by using stable isotope-labeled tracers, and will be discussed in detail. The methodology has been in use for many years and is constantly being refined. A variety of tracers and analytical approaches are available and can be used; knowing the advantages, assumptions and limitations of each is essential for the planning of studies and the interpretation of data, which may provide unique insights into human lipid metabolism.

引用

页码：215 / 230

页数：16

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