Do Montelukast Sodium and N-Acetylcysteine Have a Nephroprotective Effect on Unilateral Ureteral Obstruction? A Placebo Controlled Trial in a Rat Model

被引:7
作者
Sunay, Melih [1 ]
Karakan, Tolga [1 ]
Aydin, Arif [1 ]
Koca, Gokhan [2 ]
Borcek, Pinar [3 ]
Ogus, Elmas [4 ]
机构
[1] Ankara Numune Training & Res Hosp, Minist Hlth, Urol Clin, Ankara, Turkey
[2] Ankara Numune Training & Res Hosp, Minist Hlth, Dept Nucl Med, Ankara, Turkey
[3] Ankara Numune Training & Res Hosp, Minist Hlth, Dept Pathol, Ankara, Turkey
[4] Ankara Numune Training & Res Hosp, Minist Hlth, Dept Biochem, Ankara, Turkey
关键词
kidney; ureteral obstruction; oxidative stress; montelukast; acetylcysteine; ISCHEMIA/REPERFUSION INJURY; HEPATORENAL-SYNDROME; RECEPTOR ANTAGONIST; RENAL-FUNCTION; PROTECTS; DAMAGE; EXPRESSION; PREVENTION; SERUM;
D O I
10.1016/j.juro.2015.03.003
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Purpose: We assessed the nephroprotective effects of montelukast sodium and N-acetylcysteine on secondary renal damage due to unilateral ureteral obstruction in a rat model. Materials and Methods: In this study 30 Wistar albino male rats were randomized into 3 groups, including placebo, N-acetylcysteine and montelukast sodium. Three rats served as the control group. The left ureter of the rats was sutured with 4-zero polyglactin sutures. Medications were given 3 days before obstruction and continued for 15 days. Dimercaptosuccinic acid renal scintigraphy was performed before obstruction and on day 15. Rats were sacrificed on day 15 and histopathological examinations were done. We biochemically assessed oxidative stress markers (myeloperoxidase and malondialdehyde), sulfhydryl and total nitrite for lipid peroxidation, oxidative protein damage and antioxidant levels, respectively. Results: On pathological examination inflammation and tubular epithelial damage in the N-acetylcysteine and montelukast sodium groups were less than in the placebo group (p < 0.05). No difference was seen in normal kidneys. Myeloperoxidase, malondialdehyde and total nitrite levels in the N-acetylcysteine group, and myeloperoxidase and malondialdehyde levels in the montelukast sodium group were lower than in the placebo group (p < 0.05). No statistical difference was seen in sulfhydryl levels (p > 0.05) or among the N-acetylcysteine, montelukast sodium and placebo groups on scintigraphy (p > 0.05). No pathological, chemical and scintigraphic differences were seen among the N-acetylcysteine, montelukast sodium and sham treated groups (p > 0.05). Conclusions: N-acetylcysteine and montelukast sodium have a protective effect against obstructive damage of the kidney. However, further investigations are needed.
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收藏
页码:1132 / 1137
页数:6
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