The Effects of Apigenin on the Expression of Fas/FasL Apoptotic Pathway in Warm Liver Ischemia-Reperfusion Injury in Rats

被引：38

作者：

Tsalkidou, Evanthia G. ^{[1
,2
]}

Tsaroucha, Alexandra K. ^{[1
,2
]}

Chatzaki, Ekaterini ^{[3
]}

Lambropoulou, Maria ^{[4
]}

Papachristou, Fotini ^{[2
]}

Trypsianis, Gregory ^{[5
]}

Pitiakoudis, Michael ^{[1
,2
]}

Vaos, Georgios ^{[2
]}

Simopoulos, Constantinos ^{[1
,2
]}

机构：

[1] Democritus Univ Thrace, Univ Hosp Alexandroupolis, Sch Med, Dept Surg 2, Alexandroupolis 68100, Greece

[2] Democritus Univ Thrace, Univ Hosp Alexandroupolis, Sch Med, Lab Expt Surg & Surg Res, Alexandroupolis 68100, Greece

[3] Democritus Univ Thrace, Univ Hosp Alexandroupolis, Sch Med, Dept Pharmacol, Alexandroupolis 68100, Greece

[4] Democritus Univ Thrace, Univ Hosp Alexandroupolis, Sch Med, Histol Lab, Alexandroupolis 68100, Greece

[5] Democritus Univ Thrace, Univ Hosp Alexandroupolis, Sch Med, Lab Med Stat, Alexandroupolis 68100, Greece

来源：

BIOMED RESEARCH INTERNATIONAL | 2014年 / 2014卷

关键词：

MITOCHONDRIAL PERMEABILITY TRANSITION; HEPATIC ISCHEMIA; MECHANISMS; NECROSIS; FLAVONOIDS; CANCER; DEATH;

D O I：

10.1155/2014/157216

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background. The aim of this experimental study was to investigate the role of apigenin in liver apoptosis, in an experimental model of hepatic ischemia-reperfusion in rats. Materials and Methods. Forty-eight Wistar rats (apigenin and control groups), 14 to 16 weeks old and weighing 220 to 350 g, were used. They were all subjected to hepatic ischemia by occlusion of the hepatic artery and portal vein for 45 minutes and reperfusion was followed for 60, 120, and 240 minutes. Apigenin was administrated intraperitoneally. Liver tissues were used for the detection of apoptosis by TUNEL assay and caspase 3 antibodies. Expression analysis of Fas/FasL genes was evaluated by real time PCR. Results. The expression analysis of Fas and FasL genes was increasing during reperfusion (significantly in the group of 240 minutes of reperfusion). It was in the same group that apigenin decreased Fas receptor levels and inhibited apoptosis as confirmed by TUNEL assay and caspase 3 antibodies. Conclusions. The effects of apigenin in the Fas/FasL mediated pathway of apoptosis, in the hepatic ischemia-reperfusion, seem to have a protective result on the hepatic cell.

引用

页数：7

共 26 条

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