Effects of different ratios of glucose to acetate on phosphorus removal and microbial community of enhanced biological phosphorus removal (EBPR) system

In this study, the effects of different ratios of glucose to acetate on enhanced biological phosphorus removal (EBPR) were investigated with regard to the changes of intercellular polyhydroxyalkanoates (PHAs) and glycogen, as well as microbial community. The experiments were carried out in five sequencing batch reactors (SBRs) fed with glucose and/or acetate as carbon sources at the ratios of 0: 100 %, 25: 75 %, 50: 50 %, 75: 25 %, and 100: 0 %. The experimental results showed that a highest phosphorus removal efficiency of 96.3 % was obtained with a mixture of glucose and acetate at the ratio of 50: 50 %, which should be attributed to more glycogen and polyhydroxyvalerate (PHV) transformation in this reactor during the anaerobic condition. PCR-denaturing gradient gel electrophoresis (DGGE) analysis of sludge samples taken from different anaerobic/aerobic (A/O) SBRs revealed that microbial community structures were distinctively different with a low similarity between each other.