Stability of RAPD fingerprints in potato: effect of source tissue and primers

被引：11

作者：

Chakrabarti, S. K. ^{[1
]}

Pattanayak, D. ^{[1
]}

Sarkar, D. ^{[1
]}

Chimote, V. P. ^{[1
]}

Naik, P. S. ^{[1
]}

机构：

[1] Cent Potato Res Inst, Div Crop Improvement, Shimla 171001, Himachal Prades, India

来源：

BIOLOGIA PLANTARUM | 2006年 / 50卷 / 04期

关键词：

DNA amplification; molecular marker; polymerase chain reaction (PCR); Solanum tuberosum ssp tuberosum;

D O I：

10.1007/s10535-006-0084-3

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Variations in random amplified polymorphic DNA (RAPD) profiles from leaf, stem, root, and tuber tissues were observed in case of two glasshouse grown potato cultivars using 40 decamer primers suggesting possible danger of cultivar misidentification. Genomic DNA extracted from the above four tissues of four in vitro grown potato cultivars, however, produced more uniform RAPD fingerprints. A significant effect of random primers on fingerprint uniformity was observed in case of both glasshouse and in vitro grown samples. A new concept of stability index for random primers based on homogeneity of RAPD profiles obtained from different tissues of a single plant have been introduced. It is concluded that RAPD analysis of genomic DNA extracted from any tissue of in vitro grown potato plants using 14 selected decamer primers could be used to develop RAPD fingerprints for identification of Indian potato cultivars.

引用

页码：531 / 536

页数：6

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