Potential use of flow cytometry in toxicity studies with microalgae

被引：106

作者：

Franqueira, D ^{[1
]}

Orosa, M ^{[1
]}

Torres, E ^{[1
]}

Herrero, C ^{[1
]}

Cid, A ^{[1
]}

机构：

[1] Univ A Coruna, Fac Ciencias, Lab Microbioloxia, La Coruna 15071, Spain

来源：

SCIENCE OF THE TOTAL ENVIRONMENT | 2000年 / 247卷 / 2-3期

关键词：

microalgae; aquatic toxicity test; copper; Paraquat; flow cytometry;

D O I：

10.1016/S0048-9697(99)00483-0

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Cytotoxic effects of aquatic pollutants on microalgae are very heterogeneous, and they are influenced by environmental conditions and the test species. Stress produced by copper or paraquat addition to the culture medium of two microalgae was analysed by flow cytometry. Parameters assayed were: cell volume, chlorophyll a fluorescence and cell viability. The variety of results obtained in the present study reveals that flow cytometry is a useful tool in the toxicity tests with microalgae, both marine and freshwater species, and for different kind of pollutants. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：119 / 126

页数：8

共 35 条

[1]

ABALDE J, 1995, B ENVIRON CONTAM TOX, V54, P317

[2]

Blaise Christian R., 1993, P83

[3] DIFFERENTIAL TOXICOLOGICAL RESPONSE TO CADMIUM IN ANABAENA STRAIN PCC-7119 GROWN WITH NO3- OR NH4+ AS NITROGEN-SOURCE [J].

BOLANOS, L ;

GARCIAGONZALEZ, M ;

MATEO, P ;

BONILLA, I .

JOURNAL OF PLANT PHYSIOLOGY, 1992, 140 (03) :345-349

[4] ULTRASTRUCTURE OF CHLAMYDOMONAS-REINHARDTII FOLLOWING EXPOSURE TO PARAQUAT - COMPARISON OF WILD-TYPE AND A PARAQUAT-RESISTANT MUTANT [J].

BRAY, DF ;

BAGU, JR ;

NAKAMURA, K .

CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1993, 71 (01) :174-182

[5]

Butler WL, 1977, ENCYCLOPEDIA PLANT P, V5, P149

[6] COPPER TOXICITY ON THE MARINE MICROALGA PHAEODACTYLUM-TRICORNUTUM - EFFECTS ON PHOTOSYNTHESIS AND RELATED PARAMETERS [J].

CID, A ;

HERRERO, C ;

TORRES, E ;

ABALDE, J .

AQUATIC TOXICOLOGY, 1995, 31 (02) :165-174

[7]

Cid A, 1996, CYTOMETRY, V25, P32, DOI 10.1002/(SICI)1097-0320(19960901)25:1<32::AID-CYTO4>3.3.CO

[8]

2-A

[9] EFFECTS OF COPPER AND ZINC ON GROWTH, MORPHOLOGY, AND METABOLISM OF ASTERIONELLA-JAPONICA (CLEVE) [J].

FISHER, NS ;

JONES, GJ ;

NELSON, DM .

JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY, 1981, 51 (01) :37-56

[10] A comparison of the relative sensitivity of structural and functional cellular responses in the alga Chlamydomonas eugametos exposed to the herbicide paraquat [J].

Franqueira, D ;

Cid, A ;

Torres, E ;

Orosa, M ;

Herrero, C .

ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1999, 36 (03) :264-269

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