Potential use of flow cytometry in toxicity studies with microalgae

被引：104
|
作者：
Franqueira, D [1 ]
Orosa, M [1 ]
Torres, E [1 ]
Herrero, C [1 ]
Cid, A [1 ]
机构：
[1] Univ A Coruna, Fac Ciencias, Lab Microbioloxia, La Coruna 15071, Spain
来源：
SCIENCE OF THE TOTAL ENVIRONMENT | 2000年 / 247卷 / 2-3期
关键词：
microalgae; aquatic toxicity test; copper; Paraquat; flow cytometry;
D O I：
10.1016/S0048-9697(99)00483-0
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Cytotoxic effects of aquatic pollutants on microalgae are very heterogeneous, and they are influenced by environmental conditions and the test species. Stress produced by copper or paraquat addition to the culture medium of two microalgae was analysed by flow cytometry. Parameters assayed were: cell volume, chlorophyll a fluorescence and cell viability. The variety of results obtained in the present study reveals that flow cytometry is a useful tool in the toxicity tests with microalgae, both marine and freshwater species, and for different kind of pollutants. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：119 / 126
页数：8
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