The kinetics of hydrolysis of animal fat by pancreatic lipase

Hydrolysis of lipids from beef fat by pancreatic lipase was studied. The maximum release of free fatty acids was shown to occur at 40degreesC for the first 3 h of the experiment. After this, transetherification was predominant. The main kinetic parameters were the following: maximum hydrolysis rate, V = 1.25 +/- 0.1 mg fat/ml min; Michaelis constant, K-M(H) = 100 +/- 12 mg fat/ml; constant of substrate inhibition, K-S = 10.0 +/- 0.8 mg fat/ml; equilibrium constant, K-p = 277 +/- 170 mg fat/ml; and activation energy of beef fat hydrolysis by pancreatic lipase, E-a = 19.1 +/- 1.1 kJ/mole. The kinetic method used could be applied to development of the method for biotransformation of poorly assimilated fats into more valuable products.