Ectopic Expression of CrPIP2;3, a Plasma Membrane Intrinsic Protein Gene from the Halophyte Canavalia rosea, Enhances Drought and Salt-Alkali Stress Tolerance in Arabidopsis

被引:22
|
作者
Zheng, Jiexuan [1 ,2 ,3 ]
Lin, Ruoyi [1 ,2 ,3 ]
Pu, Lin [1 ,2 ,3 ]
Wang, Zhengfeng [4 ,5 ]
Mei, Qiming [4 ,5 ]
Zhang, Mei [1 ,2 ,6 ]
Jian, Shuguang [1 ,2 ,4 ,5 ,7 ]
机构
[1] Chinese Acad Sci, South China Bot Garden, Guangdong Prov Key Lab Appl Bot, Guangzhou 510650, Peoples R China
[2] Chinese Acad Sci, South China Bot Garden, Key Lab South China Agr Plant Mol Anal & Genet Im, Guangzhou 510650, Peoples R China
[3] Univ Chinese Acad Sci, Beijing 100039, Peoples R China
[4] Chinese Acad Sci, Ctr Plant Ecol, Core Bot Gardens, Key Lab Vegetat Restorat & Management Degraded Ec, Guangzhou 510650, Peoples R China
[5] Southern Marine Sci & Engn Guangdong Lab Guangzho, Guangzhou 511458, Peoples R China
[6] Chinese Acad Sci, Core Bot Gardens, Ctr Econ Bot, Guangzhou 510650, Peoples R China
[7] Chinese Acad Sci, South China Bot Garden, CAS Engn Lab Vegetat Ecosyst Restorat Isl & Coast, Guangzhou 510650, Peoples R China
关键词
plasma membrane intrinsic proteins; salt-alkali; drought; Canavalia rosea; AQUAPORIN ZMPIP2/5; ATRIPLEX-CANESCENS; WATER CHANNELS; GROWTH-RATE; TRANSPORT; IMMUNITY; PIP1;
D O I
10.3390/ijms22020565
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Aquaporins are channel proteins that facilitate the transmembrane transport of water and other small neutral molecules, thereby playing vital roles in maintaining water and nutrition homeostasis in the life activities of all organisms. Canavalia rosea, a seashore and mangrove-accompanied halophyte with strong adaptability to adversity in tropical and subtropical regions, is a good model for studying the molecular mechanisms underlying extreme saline-alkaline and drought stress tolerance in leguminous plants. In this study, a PIP2 gene (CrPIP2;3) was cloned from C. rosea, and its expression patterns and physiological roles in yeast and Arabidopsis thaliana heterologous expression systems under high salt-alkali and high osmotic stress conditions were examined. The expression of CrPIP2;3 at the transcriptional level in C. rosea was affected by high salinity and alkali, high osmotic stress, and abscisic acid treatment. In yeast, the expression of CrPIP2;3 enhanced salt/osmotic and oxidative sensitivity under high salt/osmotic and H2O2 stress. The overexpression of CrPIP2;3 in A. thaliana could enhance the survival and recovery of transgenic plants under drought stress, and the seed germination and seedling growth of the CrPIP2;3 OX (over-expression) lines showed slightly stronger tolerance to high salt/alkali than the wild-type. The transgenic plants also showed a higher response level to high-salinity and dehydration than the wild-type, mostly based on the up-regulated expression of salt/dehydration marker genes in A. thaliana plants. The reactive oxygen species (ROS) staining results indicated that the transgenic lines did not possess stronger ROS scavenging ability and stress tolerance than the wild-type under multiple stresses. The results confirmed that CrPIP2;3 is involved in the response of C. rosea to salt and drought, and primarily acts by mediating water homeostasis rather than by acting as an ROS transporter, thereby influencing physiological processes under various abiotic stresses in plants.
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页码:1 / 20
页数:20
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