Electrochemiluminescence Microscopy of Cells: Essential Role of Surface Regeneration

被引：41
作者：
Han, Dongni [1 ,2 ,3 ]
Goudeau, Bertrand [1 ]
Jiang, Dechen [4 ,5 ]
Fang, Danjun [2 ,3 ]
Sojic, Neso [1 ,6 ]
机构：
[1] Univ Bordeaux, Bordeaux INP, CNRS, UMR 5255, Site ENSCBP, F-33607 Pessac, France
[2] Nanjing Med Univ, Collaborat Innovat Ctr Cardiovasc Dis Translat Me, Sch Pharm, Nanjing 211126, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Collaborat Innovat Ctr Cardiovasc Dis Translat Me, Key Lab Targeted Intervent Cardiovasc Dis, Nanjing 211126, Jiangsu, Peoples R China
[4] Nanjing Univ, State Key Lab Analyt Chem Life, Nanjing 210093, Jiangsu, Peoples R China
[5] Nanjing Univ, Sch Chem & Chem Engn, Nanjing 210093, Jiangsu, Peoples R China
[6] South Ural State Univ, Dept Chem, Chelyabinsk 454080, Russia
来源：
ANALYTICAL CHEMISTRY | 2021年 / 93卷 / 03期
关键词：
D O I：
10.1021/acs.analchem.0c05123
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Electrochemiluminescence (ECL) microscopy is successfully applied to image cells, micro-/nano-objects, and electrochemical processes at electrode surfaces. The classic ECL tandem system is composed of the [Ru(bpy)(3)](2+) luminophore with the very efficient tripropylamine (TPA) coreactant. The dramatic decrease of the ECL signal observed when recording successive ECL images constitutes a key limitation for the development of ECL microscopy. Herein, we investigated the progressive decrease of the ECL signal of Chinese hamster ovary (CHO) cells. The plasma membranes of CHO cells were labeled with a [Ru(bpy)(3)](2+ )derivative, and the ECL images were recorded using the TPA coreactant. We demonstrate that the loss of the ECL signal is related to the electrochemical step because of a progressive lower TPA oxidation current. We tested a cathodic regenerative treatment of the electrode surface, which allowed us to restore the initial TPA oxidation intensity and thus to record a sequence of ECL images without any vanishing of the light signal. The electrochemical approach presented here is an essential step for the development of ECL microscopy of cells.
引用
收藏
页码：1652 / 1657
页数：6
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