The Protective Effects of Alisol A 24-Acetate from Alisma canaliculatum on Ovariectomy Induced Bone Loss in Vivo

被引:24
作者
Hwang, Yun-Ho [1 ]
Kang, Kyung-Yun [1 ]
Lee, Sung-Ju [1 ]
Nam, Sang-Jip [2 ]
Son, Young-Jin [1 ]
Yee, Sung-Tae [1 ]
机构
[1] Sunchon Natl Univ, Dept Pharm, 255 Joongang Ro, Sunchon 549742, South Korea
[2] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea
基金
新加坡国家研究基金会;
关键词
osteoporosis; bone loss; alisol A 24-acetate; Alisma canaliculatum; regulator T cell; traditional Korean medicine; REGULATORY T-CELLS; ESTROGEN DEFICIENCY; RATS; OSTEOPOROSIS; EXTRACT; REPLACEMENT; WOMEN; MICE;
D O I
10.3390/molecules21010074
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Alisma canaliculatum is a herb commonly used in traditional Korean medicine, and has been shown in scientific studies to have antitumor, diuretic hepatoprotective, and antibacterial effects. Recently, the anti-osteoclastogenesis of alisol A 24-acetate from Alisma canaliculatum was investigated in vitro. However, the influence of alisol A 24-acetate on osteoporosis in animals has not been investigated. The present study was undertaken to investigate the anti-osteoporotic effect of alisol A 24-acetate on bone mass in ovariectomized (OVX) mice and to identify the mechanism responsible for its effects. OVX mice were treated daily with 0.5 or 2 g/g of alisol A 24-acetate for a period of six weeks. It was found that these administrations significantly suppressed osteoporosis in OVX mice and improved bone morphometric parameters. The serum estradiol, bone alkaline phosphatase levels, regulatory T/Th17 cell numbers were significantly increased by alisol A 24-acetate as compared with untreated OVX mice. In addition, TRAP activity was inhibited by alisol A 24-acetate in OVX mice. These results suggest alisol A 24-acetate effectively prevents bone loss in OVX mice, and that it can be considered a potential therapeutic for the treatment of postmenopausal osteoporosis.
引用
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页数:11
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