Modulatory Role of Riboceine and Vitamin C Supplementation on Altered Oxidative Stress Status of Alloxan-induced Diabetic Wistar Rats

被引：1

作者：

Thomas, Akingbade Bamidele ^{[1
]}

Emeke, Ojieh Anthony ^{[2
]}

Chukwuebuka, Nwogueze Bartholomew ^{[3
]}

Oghenerukevwe, Omeru ^{[2
]}

Ikemefune, Ebuwa Emmanuel ^{[2
]}

机构：

[1] Cent Hosp, Dept Physiotherapy, Benin, Edo State, Nigeria

[2] Delta State Univ, Dept Human Physiol, Abraka, Delta State, Nigeria

[3] Evangel Univ, Dept Human Physiol, Akaeze, Ebonyi State, Nigeria

来源：

BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS | 2020年 / 13卷 / 04期

关键词：

RIBOCEINE; VITAMIN C; DIABETES MELLITUS; OXIDATIVE STRESS; ALLOXAN; ANTIOXIDANTS; CYSTEINE; ASSAY;

D O I：

10.21786/bbrc/13.4/75

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This study examined the anti-oxidative effects of Riboceine and vitamin C supplementation in the amelioration of tissue damage that results from oxidative stressors in diabetic rats. Twenty-five (25) mature Wistar rats (150180kg) were used for the study. They were randomly assigned into five (5) groups of five rats each: Normal Control (NC), Diabetic Mellitus(DM) (induced by Alloxan at 120mg/kg body weight i.p), Diabetic rats treated with 30mg/kg body weight of RiboCeine (DM+RibCys), Diabetic rats treated with 125 mg/kg body weight of Vitamin C (DM+VitC) and Diabetic rats treated with 30 mg/kg body weight of RiboCeine + 125mg/kg body weight of Vitamin C (DM+RibCys +VitC). Following period of 20-day treatment, all the rats were sacrificed and samples of pancreas, kidney and liver tissues were collected and assayed for changes in oxidative stress biomarkers: Glutathione (GSH), Catalase (CAT), Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), Glutathione s-transferase (GST) and Malondialdehyde (MDA) levels. The tissues were also examined for Histo-architectural changes. Data were analysed using One Way Analysis of Variance (ANOVA) followed by Fisher's Least Significant Difference (LSD). A p-value of p < 0.05 was considered statistically significant. RibCys and DM+RibCys+VitC treated rats, showed statistically significant decrease in hyperglycaemia. However, RibCys dosed rats, showed no statistically significant effect. The DM rats showed statistically significant increased MDA and decreased CAT, SOD, GPx, GST levels in the tissues. The RibCys, VitC and DM+VitC+RibCys treated rats, showed statistically significant amelioration in increased MDA and decreased CAT, SOD, GPx, GST levels. Present results concluded that RibCys has ameliorative effects on diabetes induced-oxidative stress. Also, RibCys, VitC and VitC+RibCys have equally antioxidative effects on tissues characterized by biomarkers of oxidative stress.

引用

页码：2130 / 2138

页数：9

共 33 条

[1]

Adelakun SA., 2018, Current Research in Diabetes and Obesity Journal, V9, P1

[2]

AEBI H, 1984, METHOD ENZYMOL, V105, P121

[3]

Al-Enazi M.M., 2014, PHARM PHARM, V05, P876

[4]

Alsaif M. A., 2009, Pakistan Journal of Nutrition, V8, P745

[5] Amelioration of neurological and biochemical deficits by peroxynitrite decomposition catalysts in experimental diabetic neuropathy [J].

Arora, Manish ;

Kumar, Ashutosh ;

Kaundal, Ravinder K. ;

Sharma, Shyam S. .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2008, 596 (1-3) :77-83

[6]

Chatteijea MN., 2002, Textbook of medical biochemistry, P317

[7]

Chuku LC, 2020, EUROPEAN J MED PLANT, V31, P29

[8]

Cu XP, 2008, J NUTR SCI VITAMINOL, V54, P321, DOI 10.3177/jnsv.54.321

[9]

Das Evcimen N, 2004, GEN PHYSIOL BIOPHYS, V23, P347

[10]

Dawud F. A., 2012, Current Research Journal of Biological Sciences, V4, P123

← 1 2 3 4 →