A robust and fast bacteria counting method using CdSe/ZnS core/shell quantum dots as labels

被引：14

作者：

Fu, Xin ^{[1
]}

Huang, Kelong ^{[1
]}

Liu, Suqin ^{[1
]}

机构：

[1] Cent S Univ, Dept Chem & Chem Engn, Changsha 410083, Peoples R China

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2009年 / 79卷 / 03期

关键词：

CdSe/ZnS nanocrystals; Escherichia coli; Fluorescence measurement; NANOCRYSTALS;

D O I：

10.1016/j.mimet.2009.09.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, sensitive fluorescence measurement method for detecting the bacterial count using CdSe/ZnS as a fluorescence marker was described. High-quality CdSe/ZnS nanocrystals were synthesized and successfully conjugated with bacteria. The fluorescence intensity was proportional to bacterial count in the range of 10(2)-10(8) CFU/mL and the low detection limit was 10(2) CFU/mL. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：367 / 370

页数：4

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