Whole Brain Imaging with Serial Two-Photon Tomography

被引：48
作者：
Amato, Stephen P. [1 ]
Pan, Feng [2 ]
Schwartz, Joel [1 ]
Ragan, Timothy M. [1 ]
机构：
[1] TissueVision Inc, Cambridge, MA USA
[2] Pfizer Inc, Cambridge, MA USA
来源：
FRONTIERS IN NEUROANATOMY | 2016年 / 10卷
关键词：
serial two-photon tomography; serial section tomography; brain mapping; neural circuits; Alzheimer's disease; LARGE TISSUE SAMPLES; MULTIPHOTON MICROSCOPY; MOUSE-BRAIN; IN-VIVO; TRANSGENIC MICE; FLUORESCENCE; ACTIVATION; VISUALIZATION; FIXATION; CIRCUITS;
D O I：
10.3389/fnana.2016.00031
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
Imaging entire mouse brains at submicron resolution has historically been a challenging undertaking and largely confined to the province of dedicated atlasing initiatives. This has limited systematic investigations into important areas of neuroscience, such as neural circuits, brain mapping and neurodegeneration. In this article, we describe in detail Serial Two-Photon (STP) tomography, a robust, reliable method for imaging entire brains with histological detail. We provide examples of how the basic methodology can be extended to other imaging modalities, such as Optical Coherence Tomography (OCT), in order to provide unique contrast mechanisms. Furthermore, we provide a survey of the research that STP tomography has enabled in the field of neuroscience, provide examples of how this technology enables quantitative whole brain studies, and discuss the current limitations of STP tomography-based approaches.
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页数：11
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