Distinct population of highly malignant cells in a head and neck squamous cell carcinoma cell line established by xenograft model

被引:21
作者
Chen, Chi-Yuan [1 ]
Chiou, Shih-Hwa [2 ]
Huang, Chih-Yang [3 ,4 ,5 ,6 ]
Jan, Chia-Ing [7 ]
Lin, Shu-Chun [1 ]
Tsai, Ming-Long [2 ]
Lo, Jeng-Fan [1 ]
机构
[1] Natl Yang Ming Univ, Inst Oral Biol, Taipei 112, Taiwan
[2] Taipei Vet Gen Hosp, Dept Med Res & Educ, Taipei, Taiwan
[3] China Med Univ, Grad Inst Chinese Med Sci, Taichung, Taiwan
[4] China Med Univ, Inst Med Sci, Taichung, Taiwan
[5] China Med Univ, Inst Basic Med Sci, Taichung, Taiwan
[6] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung, Taiwan
[7] China Med Univ & Hosp, Dept Pathol, Taichung, Taiwan
关键词
CANCER STEM-CELLS; BMI-1; ONCOPROTEIN; BREAST-CANCER; SELF-RENEWAL; PROSPECTIVE IDENTIFICATION; EPITHELIAL-CELLS; TUMOR-SUPPRESSOR; EXPRESSION; PATHWAY; SUBPOPULATION;
D O I
10.1186/1423-0127-16-100
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The progression and metastasis of solid tumors, including head and neck squamous cell carcinoma (HNSCC), have been related to the behavior of a small subpopulation of cancer stem cells. Here, we have established a highly malignant HNSCC cell line, SASVO3, from primary tumors using three sequential rounds of xenotransplantation. SASVO3 possesses enhanced tumorigenic ability both in vitro and in vivo. Moreover, SASVO3 exhibits properties of cancer stem cells, including that increased the abilities of sphere-forming, the number of side population cells, the potential of transplanted tumor growth and elevated expression of the stem cell marker Bmi1. Injection of SASVO3 into the tail vein of nude mice resulted in lung metastases. These results are consistent with the postulate that the malignant and/or metastasis potential of HNSCC cells may reside in a stem-like subpopulation.
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页数:11
相关论文
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