Costimulation blockade by combining CTLA4Ig with anti-CD40L mAb markedly inhibits the inflammatory response of experimental autoimmune myocarditis

被引：6

作者：

Chen, Qing-Quan ^{[1
]}

Chen, Min ^{[1
]}

Zhang, Li-Hua ^{[2
]}

Zeng, Yu ^{[1
]}

Liu, Qi-Cai ^{[1
,3
]}

Yang, Xiu-Lin ^{[1
]}

Lin, Xu-Chen ^{[1
]}

机构：

[1] Fujian Med Univ, Dept Lab Med, Med Technol & Engn Coll, 88 Jiaotong Rd, Fuzhou 350004, Fujian, Peoples R China

[2] Fujian Med Univ, Dept Lab Med, Affiliated Union Hosp, Fuzhou, Fujian, Peoples R China

[3] Fujian Med Univ, Dept Lab Med, Affiliated Hosp 1, Fuzhou, Fujian, Peoples R China

来源：

EUROPEAN JOURNAL OF INFLAMMATION | 2017年 / 15卷 / 01期

基金：

中国国家自然科学基金;

关键词：

anti-CD40L mAb; autoimmune myocarditis; costimulation blockade; CTLA4Ig; MICE;

D O I：

10.1177/1721727X16686980

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The aim of this study was to investigate the effect of costimulation blockade with cytotoxic T-lymphocyte-associated-antigen 4-immunoglobulin (CTLA4Ig) and anti-CD40L monoclonal antibody (anti-CD40L mAb) on an experimental autoimmune myocarditis (EAM) mouse model. Characteristics of myocardial tissue were observed by hematoxylin and eosin (H&E) staining. The messenger RNA (mRNA) levels of CTLA4, CD40L, IFN-gamma, and IL-4 were detected by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). Serum concentrations of IFN-gamma and IL-4 were determined by ELISA. After immune intervention, the inflammatory score, mRNA levels of CTLA4 and CD40L, and IFN-gamma level were decreased. Furthermore, these parameters in the combinational intervention group (blockade by CTLA4Ig and anti-CD40L mAb) were significantly decreased, compared to the single intervention group (blockade by CTLA4Ig or anti-CD40L mAb). However, after costimulation, blockade serum IL-4 levels were increased. Therefore, costimulation blockade by combination CTLA4Ig and anti-CD40L mAb could more effectively inhibit the inflammatory response of EAM than single use of CTLA4Ig or anti-CD40L mAb.

引用

页码：28 / 34

页数：7

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