Intestinal schistosomiasis among preschool and school-aged children in a rural setting near Alexandria: initiative for elimination

被引:6
作者
Allam, Amal Farahat [1 ]
Salem, Aziza [1 ]
Elsheredy, Amel [2 ]
Dewair, Musaad Mohammed [3 ]
Ibrahim, Heba Said [1 ]
Farag, Hoda Fahmy [1 ]
Hagras, Nancy Abd-elkader [4 ]
Shehab, Amel Youssef [1 ]
机构
[1] Univ Alexandria, Med Res Inst, Dept Parasitol, 165 El Horreya Ave, Alexandria, Egypt
[2] Univ Alexandria, Med Res Inst, Dept Microbiol, Alexandria, Egypt
[3] Misr Univ Sci & Technol Cairo, Fac Allied Med Sci, Dept Med Lab, Cairo, Egypt
[4] Pharos Univ Alexandria, Fac Allied Med Sci, Dept Med Lab, Alexandria, Egypt
关键词
Schistosoma mansoni; preschool‐ aged children; school‐ Kato‐ Katz; praziquantel; real‐ time PCR; Egypt; REAL-TIME PCR; HAEMATOBIUM; MANSONI; QUANTIFICATION; SAMPLES;
D O I
10.1111/tmi.13562
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Objective To assess the status of intestinal schistosomiasis among preschool-aged (PSAC) and school-aged children (SAC) and to compare the efficacy of praziquantel (PZQ) in both groups. Methods The study was conducted on 400 children; 103 PSAC and 297 SAC. Diagnosis of Schistosoma mansoni was based on triplicate Kato-Katz thick smears from a single stool sample. To identify the missed cases by Kato-Katz, 120 randomly selected negative cases (38 PSAC and 82 SAC) were screened by real-time PCR. All S. mansoni-positive cases by Kato-Katz were treated by crushed PZQ tablets. Four weeks after treatment, the cure rate was assessed by Kato-Katz smears and real-time PCR. Results The prevalence of S. mansoni with Kato-Katz was 7.8% among PSAC and 7.4% among SAC. Most of children (63.3%) had light-intensity infection. The cure rate was 100% among PSAC by both techniques, and 91%, and 77.2% among SAC by Kato-Katz and real-time PCR, respectively. In the 120 stool samples screened by real-time PCR, S. mansoni prevalence was 25%; 15.8% and 29.3% were among PSAC and SAC respectively. Treated cases showed a lower range of Ct values than untreated cases. Two melting temperature ranges (Tm = 83-87 degrees C and 89-93 degrees C) were recognised among uncured cases which may point to S. mansoni genetic variability. Conclusion Continuous monitoring and inclusion of PSAC in schistosomiasis control programmes are crucial. Real-time PCR and other molecular tools are recommended for evaluation of the true prevalence, assessment of cure and further studies on genetic diversity.
引用
收藏
页码:632 / 639
页数:8
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