A novel disposable electrochemical DNA biosensor for the rapid detection of Bacillus thuringiensis

被引：17

作者：

Raju, V. Manohar ^{[1
,2
]}

Bhavana, V. ^{[1
,2
]}

Gayathri, G. K. ^{[3
]}

Suryan, Sandeep ^{[1
,2
]}

Reddy, Roopa ^{[1
]}

Reddy, Narendra ^{[1
]}

Ravikumar, C. R. ^{[4
]}

Santosh, Mysore Sridhar ^{[1
]}

机构：

[1] Ctr Incubat Innovat Res & Consultancy CIIRC, Jyothy Inst Technol, Off Kanakapura Rd, Bengaluru 560082, Karnataka, India

[2] Visvesvaraya Technol Univ VTU, Belagavi 590018, Karnataka, India

[3] PES Univ, Dept Biotechnol, Outer Ring Rd,BSK 3rd Stage, Bengaluru 560085, Karnataka, India

[4] East West Inst Technol, Dept Sci, Res Ctr, Bangalore 560091, Karnataka, India

来源：

MICROCHEMICAL JOURNAL | 2020年 / 159卷

关键词：

Bacillus thuringiensis; Cry1; gene; DNA biosensor; Methylene blue; DNA hybridization; METHYLENE-BLUE; GOLD ELECTRODES; ANTHRACIS; CEREUS; TOXINS; LABEL; PCR; POLYNUCLEOTIDES; IMMOBILIZATION; NANOCOMPOSITE;

D O I：

10.1016/j.microc.2020.105434

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A custom designed DNA probe against cry1 gene of Bacillus thuringiensis has been used to fabricate a DNA biosensor by self-assembled monolayers of mercaptohexanol (MCH) and thiol-linked custom designed probe (ssDNA-thiol), immobilized on a screen printed gold nanostructured electrode. DNA immobilization and hybridization have been characterized by differential pulse voltammetry (DPV) using methylene blue as an electrochemical hybridization indicator. With this approach, the target DNA could be quantified in the range of 1.0 x 10(-6) M to 1.0 x 10(-12) M, with a detection limit of 0.997 x 10(-12) M by 3 sigma. Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) have been performed to characterize the sequentially modified electrode. In addition, a non-complementary DNA sequence of Bacillus cereus has been tested as a non-target, that hardly causes any variation in the reduction current of MB reaffirming that the DNA-based biosensor is high selective to Bacillus thuringiensis. The electrochemical detection methods employed in this work can be extended further to develop DNA-based biosensors for the detection of other microorganisms having applications in agriculture, food industries and medical applications.

引用

页数：8

共 68 条

[1] Rapid and Sensitive Detection of Foodborne Pathogenic Bacteria (Staphylococcus aureus) Using an Electrochemical DNA Genomic Biosensor and Its Application in Fresh Beef [J].