Magnetic biosensor for the detection of Yersinia pesos

被引：70
作者：
Meyer, Martin H. F.
Stehr, Matthias
Bhuju, Sabin
Krause, Hans-Joachim
Hartmann, Markus
Miethe, Peter
Singh, Mahavir
Keusgen, Michael
机构：
[1] Univ Marburg, Inst Pharmaceut Chem, D-35032 Marburg, Germany
[2] German Res Ctr Biotechnol, Dept Genome Anal, D-38124 Braunschweig, Germany
[3] LIONEX Diagnost & Therapeut GmbH, D-38124 Braunschweig, Germany
[4] Inst Thin Films & Interfaces, ISG 2, Res Ctr, Julich, Germany
[5] SENOVA GmbH, D-07745 Jena, Germany
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2007年 / 68卷 / 02期
关键词：
Yersinia pestis; antiYPF1; magnetic detection; magnetic beads;
D O I：
10.1016/j.mimet.2006.08.004
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel type of magnetic-beads based magnetic biosensor is described for the detection of Yersinia pestis. Experiments were performed with the antigen fraction F1 of these bacteria. The magnetic sensor platform offers easy and reliable detection of Y. pestis by the use of magnetic beads for labelling and quantification in a single step due to their paramagnetic features. The system uses antiYPF1 antibodies as capture element on ABICAP (R) columns as core element of the magnetic sensor. Several immobilization methods for antibodies on polyethylene were exploited. The established biosensor has a linear detection range of 25-300 ng/ml Y, pestis antigen F1 and a detection limit of 2.5 ng/ml in buffer and human blood serum. The presented sensor system is small, simple, portable and therefore usable as off-lab detection unit for medical and warfare analytes. (c) 2006 Published by Elsevier B.V.
引用
收藏
页码：218 / 224
页数：7
相关论文
共 24 条
[1]   Intraspecific diversity of Yersinia pestis [J].
Anisimov, AP ;
Lindler, LE ;
Pier, GB .
CLINICAL MICROBIOLOGY REVIEWS, 2004, 17 (02) :434-+
[2]   Detection of biomolecular interaction between biotin and streptavidin on a self-assembled monolayer using magnetic nanoparticles [J].
Arakaki, A ;
Hideshima, S ;
Nakagawa, T ;
Niwa, D ;
Tanaka, T ;
Matsunaga, T ;
Osaka, T .
BIOTECHNOLOGY AND BIOENGINEERING, 2004, 88 (04) :543-546
[3]  
Benner GE, 1999, INFECT IMMUN, V67, P1922
[4]   Simultaneous measurement of specific serum IgG responses to five select agents [J].
Biagini, RE ;
Sammons, DL ;
Smith, JP ;
MacKenzie, BA ;
Striley, CAF ;
Robertson, SA ;
Snawder, JE ;
Quinn, CP .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2005, 382 (04) :1027-1034
[5]   DETECTION OF YERSINIA-PESTIS FRACTION-1 ANTIGEN WITH A FIBER OPTIC BIOSENSOR [J].
CAO, LK ;
ANDERSON, GP ;
LIGLER, FS ;
EZZELL, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (02) :336-341
[6]   Real-time PCR assays targeting a unique chromosomal sequence of Yersinia pestis [J].
Chase, CJ ;
Ulrich, MP ;
Wasieloski, LP ;
Kondig, JP ;
Garrison, J ;
Lindler, LE ;
Kulesh, DA .
CLINICAL CHEMISTRY, 2005, 51 (10) :1778-1785
[7]   LcrV capture enzyme-linked immunosorbent assay for detection of Yersinia pestis from human samples [J].
Gomes-Solecki, MJC ;
Savitt, AG ;
Rowehl, R ;
Glass, JD ;
Bliska, JB ;
Dattwyler, RJ .
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2005, 12 (02) :339-346
[8]   Autonomous detection of aerosolized biological agents by multiplexed immunoassay with polymerase chain reaction confirmation [J].
Hindson, BJ ;
McBride, MT ;
Makarewicz, AJ ;
Henderer, BD ;
Setlur, US ;
Smith, SM ;
Gutierrez, DM ;
Metz, TR ;
Nasarabadi, SL ;
Venkateswaran, KS ;
Farrow, SW ;
Colston, BW ;
Dzenitis, JM .
ANALYTICAL CHEMISTRY, 2005, 77 (01) :284-289
[9]  
KRAUSE HJ, 2006, UNPUB J MAGN MAGN MA
[10]  
Meyer M. H. F., 2006, BIOSENS BIOELECTRON
123